酸生产微生物发酵的上升趋势:使用多功能生物源的创新方法的全面概述
1School of Biosciences Engineering and Technology, Vellore Institute of Technology Bhopal University, KothriKalan, Bhopal, 466114, Madhya Pradesh, India. vignesh.n@vitbhopal.ac.in.
Archives of microbiology
|June 17, 2025
概括
酸 (SA) 的生物生产面临能源需求和氧化还原失衡的挑战. 创新的发酵策略,包括共同培养和代谢工程,显著提高生物质的SA产量和生产率.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 代谢工程是代谢工程.
背景情况:
- 酸 (SA) 生物合成受到能源依赖的途径和氧化还原失衡的阻碍,限制了生产力.
- 对于SA的传统发酵过程,在实现高位数和产量方面经常面临挑战.
研究的目的:
- 审查和分析传统酸发酵中的挑战.
- 检查最近从基纤维素生物质生产SA的进展.
- 从复杂的生物资源中提供SA价值化的全面分析.
主要方法:
- 使用有氧酵母和选择性细菌进行共同培养发酵.
- 使用本地SA生产商优化发酵,并间歇添加二碳酸盐.
- 用酸和微生物菌株固定化进行料批发发酵,用于连续发酵.
- 对细菌和酵母菌株的代谢工程和理性设计方法.
主要成果:
- 共同培养发酵有利于微生物生长和SA生产.
- 间歇性的二碳酸盐添加增加了SA产量,达到3.86g L-1h-1.1.
- 料批发发酵达到12 g/L SA,固定菌株达到69 g/L,生产率为35.6 g L−1 h−1.
- 最近的进展表明,SA产量接近0.98gSA/g基质.
结论:
- 创新的发酵策略和代谢增强对于改善SA生产至关重要.
- 树脂纤维素生物质的利用为SA生产提供了一个可持续的途径.
- 进一步研究理性工程和流程优化可以克服目前SA生物合成的局限性.
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