肠道硫化物代谢调节行为和大脑生物能量学
Roshan Kumar1, Delawrence J Sykes2, Victor I Band3
1Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109.
概括
食中的硫和肠道微生物影响宿主健康. 肠道中硫化 (H2S) 清除受损,再加上高胺摄入量,会影响结肠健康和大脑功能,这表明饮食改变可以调节疾病.
科学领域:
- 主体微生物群的相互作用
- 代谢学 代谢学 代谢学
- 营养科学 营养科学
背景情况:
- 主体-微生物组接口涉及重要的代谢物交换,受饮食的影响.
- 硫化 (H2S) 是该接口的关键代谢物,由宿主和微生物代谢产生.
- 线粒体硫化基因氧降解酶 (SQOR) 排毒H2S,其缺乏导致李氏病.
研究的目的:
- 调查肠道中H2S清除受损是否有助于疾病.
- 为了确定饮食中的硫含量是否调节H2S代谢和相关病理.
- 探索肠道SQOR缺乏和 metionin摄入对宿主生理和微生物群的影响.
主要方法:
- 使用了患有肠上皮细胞特异性SQOR缺陷的小鼠模型.
- 服用抗生素以评估微生物对H2S代谢的贡献.
- 调节的食氨酸摄入量 (增加2.5倍) 以模仿动物与植物蛋白质的差异.
- 分析了结肠生物能学,肠道结构,微生物组合,血清生物标志物 (硫酸盐,体),运动活动和大脑MRI.
主要成果:
- 肠道SQOR缺陷单独扰乱了结肠生物能量,可以通过抗生素逆转,突出了微生物H2S的作用.
- 高氨酸摄入与SQOR缺乏有协同作用,恶化结肠结构并改变微生物组.
- 系统硫化物代谢受到影响,血清硫酸盐和体的增加表明了这一点.
- 鼠标的探索活动,心室体积和脑中的aquaporin 1水平降低.
结论:
- 饮食中的硫摄入量和肠道硫化物代谢在宿主-微生物界面上有动态相互作用,影响肠道健康.
- 肠道微生物H2S对宿主生理学有显著的贡献.
- 较低的食 metionin 摄入量显示出调节 H2S 相关病理的潜力.
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