在便样本中基于GC-MS的短链脂肪酸量化六种提取方法的对等评估
Huanhuan Jiang1, Qibin Li2, Yang Yang3
1Obstetrics and Gynecology Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Joint International Research Lab for Reproduction and Development of Ministry of Education of China, Chongqing Medical University, Chongqing 400010, China; Reproduction and Stem Cell Therapy Research Center of Chongqing, Chongqing 400010, China.
Journal of chromatography. A
|June 17, 2025
概括
对于便样本中的所有短链脂肪酸 (SCFA),没有单一的提取方法是理想的. 这项研究比较了六种方法,建议H3PO4-butanol和SPME用于研究中准确的SCFA量化.
科学领域:
- 微生物学 微生物学
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
背景情况:
- 短链脂肪酸 (SCFA) 是重要的微生物代谢物,调节宿主生理学,并与代谢疾病有关.
- 在便样本等复杂矩阵中精确量化SCFA需要强大的分析方法.
- 肠道微生物群的食纤维发酵是SCFA的主要来源.
研究的目的:
- 系统地评估和比较六种不同的提取方法,以分析便样本中的五种关键SCFA (酸,黄油酸,酸,酸,酸,异黄油酸).
- 为选择基于回收,可重复性和效率的最佳SCFA提取方法提供实用指南.
- 通过使用化学标准和人类便样本,评估这些方法的跨物种适用性.
主要方法:
- 气色谱-质谱法 (GC-MS) 用于SCFA分析.
- 六种提取方法进行了比较:HCl-DE,NaOH-Hexane,H3PO4-Butanol,H3PO4-EA,NaHCO3-MTBE,以及SPME.
- 分析性能是根据使用标准和人类便样本的特异性,线性,回收,精度和灵敏度进行评估的.
主要成果:
- 没有一种方法在所有SCFA中脱而出;不同的方法显示出化合物特定的优势.
- H3PO4-Butanol显示出高酸的复原率和良好的线性,而H3PO4-EA显示出高酸的复原率.
- SPME对酸的回收率很高,并且在便样本中提取了最多的酸,酸和异黄油酸的SCFA.
- 方法的应用揭示了遗漏堕胎患者的SCFA水平发生变化,表明了转化潜力.
结论:
- 由于其特殊优势,H3PO4-butanol和SPME是研究便样本中SCFA的推方法.
- 选择方法应根据研究目标平衡回收,可重复性和效率.
- 在便样本中进行SCFA分析,有可能在遗漏流产等临床条件下进行翻译性研究.
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