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一个侧根原始1(LRP1) 基因调节黄瓜果的果

Xiaozhe Kang¹, Yuanzuo Lv¹, Jinshuang Zheng¹

¹Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, 066004, China.

Plant physiology and biochemistry : PPB

|June 17, 2025

概括

黄瓜密度对于市场性至关重要,由CsLRP1.1调节. 这种通过遗传分析识别的基因影响了辅酶生物合成,并影响了水果的质量特征,为育种计划提供了目标.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

黄瓜 (Cucumis sativus) 水果的密度是影响营销能力的关键特征.
控制黄瓜形成的遗传和分子机制尚不清楚.

研究的目的:

为了确定负责调节黄瓜果密度的基因.
阐明鉴定出基因在发育和auxin通路中的分子功能.

主要方法:

通过EMS突变发生生成一个高突变体 ("mc-mu").
基于MutMap的映射用于识别因果基因 (CsLRP1).
在CRISPR/Cas9基因编辑中创建CsLRP1淘汰线.
现型分析,酵母两混合测定,qRT-PCR和生物信息分析.

主要成果:

在5号染色体上的基因CsaV3_5G036780 (CsLRP1) 被确定为密度的因果基因.
CsLRP1编码了一种参与auxin生物合成的SHI/STY家族蛋白质.
缺乏CsLRP1的植物显示密度增加,果子直径减少.
CsLRP1在物理上与CsSCZ-21相互作用,并负面调节密度,可能调节auxin信号传递.

结论:

CsLRP1是黄瓜果密度的关键负调节者.
了解CsLRP1的作用,可以了解黄瓜水果的发育和质量.
CsLRP1代表了黄瓜育种计划的有价值的目标,旨在增强水果特征.

关键词:

在CsLRP1中.黄瓜 - 黄瓜是一种黄瓜.基因局部化基因局部化基因转换是一种基因转换.的密度是的密度.

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