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人口欧弗拉提卡PeRAX2与PeANN1促进体相互作用,以调节基因表达和耐受性

Caixia Yan¹, Kaiyue Dong¹, Yinan Zhang²

¹Key Laboratory of Forest and Flower Genetics and Breeding of Ministry of Education, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing, 100083, China.

Plant physiology and biochemistry : PPB

|June 17, 2025

概括

基因PeRAX2通过减少的吸收来帮助植物耐受 (Cd2+). 压力较大的树中高调的PeRAX2降低了PeANN1的调节,限制了Cd2+的积累,并保持了植物健康.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
环境科学环境科学

背景情况:

树种类对植物修复至关重要,因为它们有可能大规模吸收 (Cd2+).
基因工程提供了一个有前途的途径,以提高树的Cd2+耐受性.
R2R3-MYB转录因子PeRAX2在调节树中Cd2+积累和耐受性的作用仍然不清楚.

研究的目的:

调查PeRAX2在调节Populus物种中 (Cd2+) 积累和耐受性的功能.
阐明PeRAX2影响Cd2+吸收和植物对压力的反应的分子机制.
评估PeRAX2对木进行基因改造的潜力,以提高植物修复能力.

主要方法:

在压力下对Populus euphratica的PeRAX2和PeANN1基因表达的分析.
通过DNA pull-down,电泳运动转移试验 (EMSA) 和酵母一混合 (Y1H) 试验,确认PeRAX2与PeANN1促进体结合.
VIGS,LUC记者测定和Populus中的过度表达过渡性测定,以验证PeRAX2对PeANN1和随后的耐受性的调节作用.

主要成果:

在P. euphratica中,应激调高了PeRAX2和调低了PeANN1的表达.
PeRAX2直接结合并抑制PeANN1.1的促进子活动.
过度表达PeRAX2限制了叶子中的Cd2+积累,而PeANN1过度表达增加了Cd2+积累,导致生长减少和光合作用受损.

结论:

在加压力木中升级的PeRAX2降低了PeANN1的调节,减少了Cd2+的吸收和积累.
这种PeRAX2介导的调节在压力下维持了植物生长,光合作用和活性氧物种 (ROS) 恒温.
这些发现为树中独特的PeRAX2功能提供了洞察力,这对基因工程植物有价值,以改善耐受性和植物修复.

关键词:

附录附录是指附件.抗氧化酶酶是一种抗氧化酶.Cd(2+) 的时间.H ((2) O ((2)) 的情况.这就是MYB.P. × canescensens 的时间表.人口欧弗拉蒂克河

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