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使用基于实验室效应的方法进行全废水毒性测试:与传统方法的比较

Matthew Johnson¹, Kambez Akrami², Nicholas D Crosbie³

¹Australian Rivers Institute, School of Environment and Science, Griffith University, Southport, Qld 4222, Australia; Environment, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Urrbrae, SA 5064, Australia.

The Science of the total environment

|June 17, 2025

概括

在体外生物测试为监测废水废水的传统毒性测试提供了一种敏感,高效的替代方案. 这些方法提供了更广泛的毒理学覆盖范围,可以纳入监管框架.

科学领域:

环境科学环境科学
生态毒理学生态毒理学
生物技术是生物技术.

背景情况:

废水处理厂 (WWTP) 的废水含有具有环境和健康风险的污染物.
传统的化学分析和体内直接毒性评估 (DTA) 在范围,成本和效率上都有局限性.
在体外生物测试为全面的毒性评估提供了一个有希望的替代方案.

研究的目的:

调查体外生物测试作为监测WWTP废水的补充方法的实用性.
将各种体外终点的敏感性和相关性与传统的DTA进行比较.
评估将体外测试纳入监管监测计划中的潜力.

主要方法:

使用一系列体外生物试验评估WWTP废水,以向细菌毒性,藻类生长抑制,酸碳化合物受体 (AhR) 活性和基因毒性等终点.
将体外结果与传统的体外DTA进行比较,特别是海膽受精试验.
分析了来自WWTP的废水,处理水平各不相同.

主要成果:

初级处理的废水对细菌具有高毒性,内分泌活性化合物含量较高.
经过消毒过程的三级处理废水表现出较低的基因毒性.
所有废水都显示出中度至强度的AhR活性和潜在的营养诱导藻类生长.
在海刺受精试验和ERα GeneBLAzer生物试验之间发现了强烈的相关性.
细菌毒性,藻类抑制,雌激素活性和基因毒性是最敏感和最相关的终点.

结论:

试管生物测试是WWTP废水监测的有效补充工具,提供更高的灵敏度和效率.
特定的体外终点 (细菌毒性,藻类影响,雌激素性,基因毒性) 提供了有价值的毒理学数据.
这些测试有可能纳入监管框架,以改善环境风险评估.

关键词:

生物测试是一种生物测试.直接的毒性评估直接的毒性评估在体外 (in vitro) 的情况下.在活体中,在活体中.潮湿的,因为它是湿的.废水是废水,废水就是废水.

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