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Updated: Sep 19, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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一个基于Tn5转移酶的系统,用于在大肠杆菌中高效的全基因组基因激活

Yuling Song1,2, Yifei Liu2,3, Qingyan Li2,4

  • 1Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China.

ACS synthetic biology
|June 17, 2025
PubMed
概括

研究人员使用Tn5转基因酶在细菌中创建了一个全基因组基因激活的新系统. 这个工具有助于理解基因功能和优化合成生物学应用的细菌菌株.

关键词:
在Tn5转移酶中.激活基因激活 基因激活进行全基因组查.抗甘氨酸的耐药性 抗甘氨酸的耐药性转化转化转化转化 转化转化

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DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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