相关实验视频

Updated: Sep 19, 2025

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

在膀癌中,LINC00525通过YAP稳定介导的转录激活驱动了激进的表型

Jiaming Liang¹, Shuyue Guo², Youzhi Wang¹

¹Tianjin institute of Urology, The Second Hospital of Tianjin Medical University, 23 Pingjiang Road, Hexi District, Tianjin, 300211, China.

Cancer cell international

|June 17, 2025

概括

长非编码RNA LINC00525通过稳定YAP,一个关键蛋白质,促进膀癌的进展. 升级LINC00525与晚期膀癌相关,并驱动瘤生长,提供潜在的治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNAs (lncRNAs) 调节基因表达,并与各种癌症有关.
目前尚不清楚LINC00525在膀癌 (BLCA) 发病过程中的具体作用.
了解ncRNA功能对于开发新型癌症疗法至关重要.

研究的目的:

研究LINC00525在膀癌中的表达和功能.
阐明LINC00525影响膀癌进展的分子机制.
探索LINC00525/YAP轴作为膀癌治疗点的潜力.

主要方法:

定量实时PCR和西部涂抹,以评估患者组织和细胞系中的LINC00525和YAP表达.
在体外测试 (扩散,迁移,入侵) 来评估LINC00525.5的功能作用.
生物信息分析和体内/体外实验以确认LINC00525/YAP相互作用和机制.

主要成果:

在膀癌组织和细胞系中,LINC00525的表达显著上调,与瘤阶段相关 (MIBC与NMIBC).
Knockdown 的 LINC00525 抑制膀癌细胞的增殖,迁移和入侵,而过度表达增强了这些过程.
LINC00525通过减少其酸化和泛化来稳定YAP,促进核进口和转录活动,从而推动瘤生长.

结论:

在膀癌中,LINC00525通过促进细胞增殖,迁移和通过YAP通路的入侵,起到瘤性lncRNA的作用.
LINC00525/YAP轴是膀癌发展和进展的关键调节器.
针对LINC00525可能是高LINC00525表达的膀癌患者的有前途的治疗策略.

关键词:

膀癌是一种癌症.这是一个LncRNARNA.在Ubiquitination中使用.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈

更多相关视频

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...