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相关概念视频

Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
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CHD7调节了人类胚胎干细胞的最终内皮和中皮发育.

Rong Hu1, Jin Zhao1, Kuan Chen Lai1

  • 1Department of Allied Health Sciences, University of Connecticut, 1390 Storrs Road, Storrs, 06269, CT, USA.

Stem cell research & therapy
|June 17, 2025
PubMed
概括

切除CHD7基因会影响人类胚胎干细胞发展成最终的内皮和中皮. 这显示出CHD7.

关键词:
查奇综合症 (CHARGE综合症) 是一种CHD7 CHD7 的时间最终的内皮体内皮质.人类胚胎干细胞的人类胚胎干细胞

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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 干细胞研究 干细胞研究

背景情况:

  • CHD7突变会导致发育障碍,如CHARGE综合征,但机制尚不清楚.
  • 胚胎最终内皮 (DE) 对于形成重要器官至关重要.
  • 了解CHD7在早期发育中的作用至关重要.

研究的目的:

  • 研究CHD7在人类胚胎干细胞 (hESC) 分化中的作用.
  • 确定CHD7删除如何影响最终内皮 (DE) 和中皮的发展.
  • 探索CHD7相关发育缺陷背后的分子机制.

主要方法:

  • 使用CRISPR/Cas9.9生成CHD7突变型 (CHD7-/-和CHD7+/-) 和野生型 (CHD7+/+) 的hESCs.
  • 在实验室中评估hESC分化为DE,中皮和外皮.
  • 进行RNA测序 (RNA-seq) 和ATAC-seq,以比较基因表达和染色质可访问性.

主要成果:

  • 删除CHD7减少了hESC分化到DE和中皮,以剂量依赖的方式.
  • 在CHD7被删除的细胞中观察到基因表达和染色质可访问性的显著变化.
  • 在CHD7删除的hESC-DE细胞中确定了40个基因的表达和可访问性下降.

结论:

  • CHD7对于hESCs的DE和中皮发育至关重要.
  • 提供了关于CHD7突变如何导致先天性异常的新见解.
  • 突出了CHD7在人类早期发育中的关键功能.