在体外结肠发酵的优化:对Flavan-3-ol代和微生物群调节的洞察
Nicole Tosi1, Leonardo Mancabelli2,3, Giulia Alessandri4
1Human Nutrition Unit, Department of Food & Drug, University of Parma, Via Volturno 39, 43125 Parma, Italy.
Journal of agricultural and food chemistry
|June 18, 2025
概括
将体外便发酵延长到48小时,可以更全面地了解肠道微生物对饮食中的多醇代谢. 这种扩展模型揭示了晚期catabolites和微生物相互作用,增强了生理学相关性.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 生物化学 生物化学
背景情况:
- 实验室便发酵模型对于研究食中flavan-3-ols的肠道微生物代谢至关重要.
- 目前的模型通常使用短发酵时间 (24小时),可能缺少完整的代谢物动力学.
研究的目的:
- 延长食多的体外便发酵时间长达48小时.
- 增强多代谢的结肠模型的生理相关性.
主要方法:
- 使用人类便泥在48小时内发酵flavan-3-ol单体 ((-) -epicatechin) 和小分子 (procyanidin B2,A2).
- 在五个时间点同时监测多催化剂,pH值和微生物组成.
- 量化十四种催化剂,以评估发酵动态.
主要成果:
- 黄-3-ol的聚合和素的结合会影响生物可访问性和代谢.
- 延长发酵 (48小时) 揭示了晚期催化剂,保持稳定的pH (5-6) 和微生物组成.
- 对微生物多样性的基质特异性影响,以及微生物种类和性催化物之间的相关性.
结论:
- 一个48小时的体外便发酵模型为研究结肠多命运提供了更大的生理相关性.
- 扩展模型对于理解肠道微生物对未消化的食多的转化更可靠.
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