相关实验视频

Updated: Sep 19, 2025

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

通过镜像间化学合成手术化学蛋白质纳米粒子疫苗

Songzi Kou¹, Chenbo Sun¹, Weitao Chen²

¹Greater Bay Biomedical Innocenter, Shenzhen Bay Laboratory, Shenzhen, China.

Angewandte Chemie (International ed. in English)

|June 18, 2025

概括

科学家们创造了一种新的方法来构建复杂的蛋白质结构,使用自然 (L) 和镜像 (D) 组件. 这种方法提高了疫苗和治疗药物的稳定性和有效性,从而导致更强的免疫反应.

科学领域:

生物化学生物化学
蛋白质工程是指蛋白质工程.
疫苗开发疫苗开发

背景情况:

镜像蛋白和核酸提供了增强的生物稳定性和生物直角性.
这些特性对于提高生物治疗药物的寿命非常有价值.

研究的目的:

开发一个模块化策略,用于构建复杂的蛋白质架构,整合L-和D-chirality.
为了创建一个性倒置连酶 (D-SpyStapler),以高效地结合D-动机.
为了证明该平台在开发先进疫苗方面的实用性.

主要方法:

开发了D-SpyStapler,一种化学合成的,性逆转的联酶.
利用L-排序酶通过聚-Gly链接器和LPXTG排序信号将D- (例如D-SpyTag,D-BDTag) 与L-蛋白结合起来.
通过使用D-SpyStapler组装D-SpyTagged和D-BDTagged的蛋白质.
构造的性仿真病毒样粒子 (VLP) 疫苗显示疟疾和冠状病毒抗原.

主要成果:

通过整合L-和D-蛋白质图案,成功地创建了奇拉化学VLP.
在使用重组蛋白或合成的疫苗设计中展示了灵活性.
奇拉混合疫苗在体外显示蛋白质分解性耐药性增加.
疫苗引起了强大的体内免疫反应.

结论:

开发的方法为制造长效疗法和疫苗提供了一个多功能平台.
这种模块化方法可以构建复杂,稳定和免疫原生物制剂.
整合D-和L-chirality为治疗和疫苗设计提供了显著的优势.

关键词:

性化学蛋白质的蛋白质是性的.镜像蛋白质是一种反射蛋白质.蛋白质纳米粒子疫苗的疫苗蛋白质溶解耐药性蛋白质溶解耐药性这是间化学.

更多相关视频

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Published on: March 16, 2022

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

相关概念视频

Chirality in Nature

Chirality in Nature

Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.

Chirality

Chirality

Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot.
Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...