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通过基于微芯片的分子检测,从阳性血液培养中检测到格拉姆阴性细菌中的β-乳酸盐抗性基因

Vittorio Ivagnes¹, Flavio De Maio², Ilaria Baccani³

¹Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.

Frontiers in cellular and infection microbiology

|June 18, 2025

概括

阿里法克斯的格拉姆阴性耐药性 (GNR) 微芯片测定准确地检测了阳性血液培养中的β-乳酸盐耐药性基因. 这种测试显示出高一致性和比较方法更广泛的基因覆盖,有助于抗微生物治疗决策.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性
分子诊断学分子诊断学

背景情况:

准确检测β-乳酸胺耐药基因对于血液感染中有效的抗菌疗法至关重要.
格拉姆阴性细菌,包括肠杆菌,Pseudomonas aeruginosa和Acinetobacter baumannii,是这些感染的重要原因.

研究的目的:

评估阿里法克斯格兰阴性耐药性 (GNR) 微芯片试验,用于直接从阳性血培养中检测β-乳酸盐耐药性基因.
评估测试的性能与既有方法和全基因组测序相比.

主要方法:

用GNR微芯片测试来测试模拟和临床格兰氏阴性阳性血液培养样本的各种β-乳酸盐耐药基因.
全基因组测序 (WGS) 作为模拟样本的参考,并为临床样本选择性测序.
生物Mérieux BioFire 血液培养识别2 (BCID2) 面板测定被用作临床样本的比较剂.

主要成果:

在模拟样本中,GNR测定在检测β-乳酸盐耐药基因方面达到99.5%的准确性.
在临床样本中观察到GNR测定和BCID2小组之间的高度一致性.
基因基因基因检测检测到额外的耐药基因 (blaCMY-2-like, blaOXA-23-like, blaSHV-ESBL) 不被BCID2覆盖,由WGS证实.

结论:

基因基因组微芯片测定表明高一致性和更广泛的β-乳酸盐耐药性基因覆盖,显示了常规诊断的潜力.
建议在更大的前性研究中进行进一步验证,以支持其临床实用性.

关键词:

在GNR微芯片测试中进行了测试.格拉姆阴性细菌是一种细菌.抗微生物耐药性抗微生物耐药性分子检测分子检测检测阳性血液培养是一种积极的血液培养.这就是β-乳酸酶.

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