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循环的树突细胞和单细胞子集的长期变化,以及创伤后过程中这些细胞上EMR2,CD97和EMR3的改变表达

Leyu Zheng¹, Carolin Fuchs¹, Christian Kleber¹

¹Research Laboratories and Department of Orthopaedics, Trauma and Plastic Surgery Leipzig University and University Hospital Leipzig Leipzig Germany.

Clinical & translational immunology

|June 18, 2025

概括

创伤损伤改变了免疫细胞群,树突细胞 (DC) 消失,单细胞子集发生变化. 粘附性GPCR EMR2表达在单细胞上增加,这表明它在受伤免疫反应中发挥了独特的作用.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
创伤研究创伤研究

背景情况:

创伤伤害释放与损伤相关的模式 (DAMP),激活免疫细胞.
树突细胞 (DC) 和单细胞对于感知DAMP和启动适应性免疫是至关重要的.
粘附GPCR EMR2 (也称为ADGRE2) 在这些免疫细胞上表达,并可能由DAMPs调节.

研究的目的:

研究创伤损伤后循环树突细胞 (DC) 和单细胞子集的动态变化.
在创伤患者中的这些免疫细胞子集上量化粘附GPCRs EMR2,CD97 (ADGRE5) 和EMR3 (ADGRE3) 的表达.
了解这些受体在应对创伤时的 (病理) 生理调节.

主要方法:

使用流细胞计,分析来自创伤患者和未受伤的志愿者的血液样本.
对各种DC和单细胞子集的EMR2,CD97和EMR3表达的量化.
免疫细胞子集组成和受体表达与损伤严重程度和临床参数的相关性.

主要成果:

创伤后常规和等离子体细胞循环中DC显著下降,与受伤严重程度相关.
单细胞子集的组成发生了变化,经典单细胞增加,非经典单细胞减少.
在所有单细胞子集上,EMR2的表达上调,而EMR3在古典单细胞 (减少) 和非古典单细胞 (增加) 上显示出不同的模式. CD97表达仍然相对稳定.
这些受体表达变化在很大程度上独立于受伤严重程度.

结论:

创伤伤害导致循环DC和单细胞群体的显著变化.
单细胞子集上EMR2,CD97和EMR3的独特调节模式表明它们在受伤免疫反应中起着特殊的作用.
这些发现为创伤后复杂的免疫适应提供了洞察力.

关键词:

在CD97 CD97 CD97 CD97 CD97 CD97 CD97 在EMR2中使用EMR2.这是EMR3的EMR3.树突细胞是一种树突细胞.单细胞子集单细胞子集创伤性伤害伤害的伤害.

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