益生菌Saccharomyces boulardii的多步路径工程,用于肠道中酸的产生
Femke Van Gaever1,2, Paul Vandecruys3,4, Yasmine Driege1,2
1VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium.
Metabolic engineering communications
|June 18, 2025
概括
工程化益生菌酵母Saccharomyces boulardii在肠道中产生酸 (ABA). 虽然在肠道中成功,但低体温阻碍了ABA的产生,需要进一步优化治疗用途.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 生物技术是生物技术.
背景情况:
- 由于其免疫和抗炎作用,酸 (ABA) 作为营养药物表现有前途.
- 目前的ABA交付方法在可扩展性,稳定性和成本方面存在局限性.
- 开发新的ABA输送系统对于其治疗应用至关重要.
研究的目的:
- 为了设计一种益生菌酵母,Saccharomyces boulardii,用于现场生产和在肠道中输送酸 (ABA).
- 为了克服与ABA短半衰期和成本效益生产相关的挑战.
- 在生理温度下研究工程S. boulardii的ABA生产.
主要方法:
- 用S. boulardii的基因工程与Botrytis cinerea的基因用于ABA生物合成.
- 通过引入细胞染色体P450减少酶基因来优化ABA生产.
- 在体内向小鼠注射工程S. boulardii,并评估肠道殖民和血清ABA水平.
- 研究生理温度 (37°C) 对ABA产生的影响.
主要成果:
- 工程S. boulardii成功地在30°C下产生了ABA,其标位明显高于之前报告的S. cerevisiae菌株.
- 益生菌在食补充剂后有效地殖民了小鼠肠道.
- 在生理老鼠体温 (37°C) 时,ABA产量显著降低,导致血清ABA水平较低.
- 对美瓦酸路径的改进显示,在37°C时有可能改善ABA产量.
结论:
- 工程设计的S. boulardii可以在肠道中产生ABA,但生理温度是有效生产的主要限制.
- 需要进一步的代谢工程来优化37°C的ABA产量,然后才能探索治疗应用.
- 这项研究为益生菌介导的ABA输送提供了一个有希望的平台,等待进一步优化.
相关概念视频
01:28Carbon-dioxide Fixation
94
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
94
01:26Respiration Pathways
221
Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
221
01:24Biosynthesis in Bacteria
122
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
122