淋巴激素通过催产素和放松素信号通路在平鱼精子生成过程中差异调节丸细胞粘附和结合综合体
Noelia López-Fortún1,2, Jose Vicente Roig-Genovés3, Ignacio Giménez3
1Institute of Marine Sciences, Spanish National Research Council (CSIC), Barcelona, Spain.
Frontiers in cell and developmental biology
|June 18, 2025
概括
化激素 (Lh) 和卵泡刺激激素 (Fsh) 通过降低细胞结合来调节塞内加尔的精子生成. 催产素 (Oxt) 和放松素 (Rln) 信号介导这种干扰,促进精子释放和荷尔蒙运输.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 鱼类生物学 鱼类生物学
背景情况:
- 精子发生的调节在远体中,特别是像塞内加尔海底 (Solea senegalensis) 这样的平鱼,是不太了解的.
- 化激素 (Lh) 触发精子生成,但精子脱落和Lh通过血液丸屏障 (BTB) 传输的机制尚不清楚.
研究的目的:
- 为了研究在塞内加尔人中基因性腺激素诱导的精子发生的分子机制.
- 阐明精子细胞如何脱离塞尔托利细胞以及Lh如何穿越BTB.
主要方法:
- 在复合卵泡刺激激素 (rFsh) 和Lh (rLh) 治疗后,RNA测序 (RNA-seq) 转录组分析丸单体.
- 在现场混合和免疫定位以确认基因和蛋白质的表达和定位.
- 在体外测试中,使用丸扩展剂与激素和受体抑制剂进行测试.
主要成果:
- 淋巴激素 (rFsh和rLh) 对细胞-细胞结合和粘附蛋白的转录进行了下调.
- 催产素 (Oxt) 和放松素 (Rln) 信号通路参与其中,它们的和受体在塞尔托利细胞和精子中表达.
- 交叉基因的Lh诱导下调是由Oxt和Rln信号介导的,涉及BTB和Sertoli-spermatid交叉的破坏.
结论:
- 催产素和放松素的信号传递通过丸单体中的淋巴激素来调节血丸屏障和塞尔托利细胞-精子结的破坏.
- 这种干扰在精子生成过程中促进了精子释放和Lh的细胞运输到精子光中.
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