相关实验视频
Updated: Sep 19, 2025

11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
27.5K
在Bombella apisCRISPR-Cas系统中的进化趋势
Carrie L Ganote1, Lílian Caesar2, Danny W Rice2
1Luddy School of Informatics, Indiana University, Bloomington, Indiana, USA.
mSystems
|June 18, 2025
概括
蜜蜂共生体Bombella apis具有动态的CRISPR-Cas免疫系统,表现出多种类型和间隔器的获取. 这些系统有助于B. apis在不同的蜜蜂殖民地中防御移动遗传元素.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 细菌和古生物利用CRISPR-Cas系统对抗菌体等外来遗传元素进行防御.
- 蜜蜂共生体Bombella apis拥有CRISPR-Cas系统,其在殖民地中的作用,包括繁殖保护和营养支持,尚未得到充分研究.
- B. apis的利基特异性可能受到不同蜂群环境中的菌体和移动遗传元素 (MGE) 压力的影响.
研究的目的:
- 研究B. apis菌株内的CRISPR-Cas类型的变异和分布.
- 分析间隔序列,了解MGE相互作用和B. apis CRISPR-Cas系统的进化历史.
- 预测不同蜜蜂微生物组成员之间的病毒序列和CRISPR系统之间的相互作用.
主要方法:
- 在B. apis菌株中对CRISPR-Cas类型和数组进行基因组分析.
- 在蜜蜂女王肠道样本中对CRISPR-Cas系统进行元基因组学分析.
- 间隔分析以推断MGE相互作用史.
- 病毒序列和CRISPR系统相互作用的生物信息预测.
主要成果:
- 在个别的B. apis基因组和蜜蜂女王肠道基因组中确定了多个CRISPR-Cas类型,其中一些具有多个阵列.
- 间隔分析揭示了菌株之间独特的序列,支持了CRISPR-Cas系统在不同的功能作用.
- 有证据表明,B. apis. 在多个基因组位置上动态获取新的间隔器,这表明B. apis.有一个灵活的抗病毒防御策略.
结论:
- B. apis CRISPR-Cas系统具有高度动态性,在蜜蜂群体内的微生物防御中发挥着功能性作用.
- 不同利基的独特间隔组合突显了MGE对利基特定的选择性压力.
- 在B. apis中CRISPR-Cas系统的灵活性质有助于其在复杂的蜜蜂微生物群中适应和生存.
相关概念视频
CRISPR and crRNAs
17.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.5K
The Antiviral System of Bacteria and Archaea: CRISPR
143
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
143
CRISPR/Cas9 Genome Editing
409
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
409
CRISPR
53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K
Cis-regulatory Sequences
10.2K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.2K
Speciation Rates
21.5K
Overview
21.5K

