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预分析条件对血型炎症生物标志物的影响

Thomas O'Donnell¹, Stephanie Weinstein¹, Yukiko Yano¹

¹Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Rockville, Maryland 20850, United States.

Journal of proteome research

|June 18, 2025

概括

血液炎症生物标志物是可靠的诊断测试. 大多数蛋白质在各种预分析条件下表现出良好的稳定性,支持它们在临床环境中的使用.

科学领域:

生物化学生物化学
临床化学临床化学
免疫学免疫学免疫学

背景情况:

基于血液的炎症生物标志物对于诊断,预后和预测测试至关重要.
分析前的加工条件可以显著影响生物标志物水平和可靠性.
血液样本处理的标准化对于一致和准确的生物标志物测量至关重要.

研究的目的:

调查针到冷时间,离心时间和管类型对炎症生物标志物的影响.
通过使用Olink Target 96炎症面板评估92种炎症生物标志物的稳定性.
评估预分析变量对与年龄相关的生物标志物协会的影响.

主要方法:

20名受试者在各种采集管 (血和血清) 中提供了21个血液样本.
使用Olink Target 96炎症面板分析了92个炎症生物标志物.
通过比较不同预分析条件的生物标志物度与参考标准,计算类内,皮尔森和斯皮尔曼相关性.

主要成果:

38%-83%的蛋白质在不同的分析前条件和血液矩阵中表现出良好的至极好的相关性.
在18种蛋白质中,测试和参考协议之间的差异很小 (>0.5NPX单位).
40%的与年龄相关的生物标志物关联在所有针到冷时间内仍然显著 (p ≤0.05).

结论:

在Olink Target 96炎症面板中的许多蛋白质在各种预分析条件下表现出强大的稳定性.
基于血液的炎症生物标志物适合在不同类型的血液样本中进行测试,支持其临床实用性.
需要进一步的研究来了解长期储存对生物标志物稳定性的影响.

关键词:

连接癌症预防研究研究癌症预防研究奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克生物样本的生物样本.生物标志物生物标志物一个血液样本.癌症癌症癌症癌症癌症流行病学研究是流行病学研究.免疫测试是一种免疫测试.这是一种炎症炎症炎症炎症.在分析前的变量.

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