线粒体蛋白Num11通过影响线粒体功能和细胞壁掩盖而对Candida albicans的致病性至关重要
Guangyuan Yang1, Xiaojia Niu1, Tian Zhuang1
1Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China.
Virulence
|June 18, 2025
概括
线粒体蛋白Num11对于Candida albicans的毒性至关重要. 它的缺失会破坏细胞分裂,线粒体功能和细胞壁完整性,导致病原性降低.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 病变的发生和发病.
背景情况:
- 线粒体定蛋白 Num1 影响真菌细胞过程.
- 它的同类分子Num11在人类病原体*Candida albicans*中的作用尚不清楚.
研究的目的:
- 在功能上描述 *Candida albicans* Num11 (CaNum11).
- 阐明CaNum11在真菌病原和宿主病原相互作用中的作用.
主要方法:
- 对*C. albicans*的删除突变分析数11.
- 细胞缺陷的评估:细胞周期,线粒体功能,细胞壁完整性.
- 在*Galleria mellonella*和小鼠模型中进行病毒性测定.
- 转录组概况和蛋白质相互作用分析.
主要成果:
- 删除Num11导致线粒体功能障碍 (聚合,ATP枯竭,ROS增加) 和细胞循环停止.
- 突变者对细胞壁剂过敏,细胞壁较厚,β-glucan/chitin暴露增加.
- Num11缺乏减弱毒性和增强巨细胞反应,与Cdc42-Cek1 MAPK通路的过度激活有关.
结论:
- Num11充当线粒体支架,维持生物能平衡,调节真菌生长.
- Num11对Cdc42-Cek1 MAPK级联进行负调节,控制细胞壁架构.
- Num11通过协调线粒体功能和细胞壁完整性,对*C. albicans*的病原发生是必不可少的.
相关概念视频
The Inner Mitochondrial Membrane
3.7K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.7K
Mitochondrial Precursor Proteins
2.6K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70 chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
Most of the mitochondrial...
2.6K
Porin Insertion in the Outer Mitochondrial Membrane
3.3K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
3.3K
ATP Synthase: Mechanism
15.3K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
15.3K
Mitochondrial Protein Sorting
4.4K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.4K
Electron Transport Chain: Complex I and II
15.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
15.1K


