储存在高温下的大豆改变了肠道质的组成,并通过增加肉的MAPK14蛋白表达增加了肠道的氧化还原平衡,破坏了肠道的氧化还原平衡
Peng Wang1, Qihui Sui1, Lili Guo1
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Poultry science
|June 18, 2025
概括
用高温储存的大豆粉 (SBM) 养肉会对生长和肠道健康产生负面影响. 储存的SBM增加了氧化应激和改变了蛋白质表达,影响肉的表现和肠道功能.
科学领域:
- 动物科学动物科学
- 禽类的营养 养
- 氧化压力生物学 氧化压力生物学
背景情况:
- 大豆粉 (SBM) 是 brojlers的主要蛋白质来源.
- 高温储存可能会降低SBM质量,可能会影响肉的健康.
- 了解这些影响对于优化家禽料配方至关重要.
研究的目的:
- 为了研究高温 (35°C) 储存的大豆粉对 brojiler生长和肠道生理学的影响.
- 阐明SBM诱导的肉变化背后的生物化学和分子机制.
- 为了识别肠道血糖组成的变化,差异表达蛋白 (DEP) 和信号通路.
主要方法:
- 用新鲜的SBM或SBM在35°C下保存2或4周的食物养192个Arbor Acres Plus肉.
- 评估生长表现 (平均每日增长,体重,料转换率).
- 测量氧化应激标记物 (蛋白质碳基,自由硫基,ROS),酶活性 (素,SOD,催化酶),肠道血糖组成,状DEP和MAPK信号通路激活.
主要成果:
- 在35°C保存4周的SBM显著降低了 brojler的平均每日增量和体重,并恶化了料转换率.
- 在高温下储存的SBM增加了氧化应激标记物 (碳нил,ROS) 和状素活性,同时降低了抗氧化酶活性 (SOD,催化酶).
- 观察到阴氨基酸水平,114个DEP (包括MAPK14) 和下游信号 (c-JUN,FoxO1) 的显著变化.
结论:
- 高温储存大大降低了大豆面粉的质量,损害了 brojler 的生长和肠道健康.
- 储存的SBM中的蛋白质氧化会诱导氧化应激,破坏肠道功能,并改变蛋白质表达特征.
- 这些发现凸显了SBM储存条件对于维持 brojler性能和肠道完整性的重要性.
相关概念视频
Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Adaptive Mechanisms in Cancer Cells
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Mismatch Repair
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Protein Denaturation
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
Amino Acid Catabolism
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...


