辐射诱导了细胞-肌纤维细胞过渡
Tomoko Yamazaki1, Kelley R Jordan1, Nathaniel Fox1
1Earle A. Chiles Research Institute, Providence Cancer Center, Portland, Oregon.
概括
辐射通过Akt信号通路促进细胞细胞成长为肌纤维细胞. 这项研究调查了辐射.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 放射治疗研究 放射治疗研究
背景情况:
- 放射治疗是癌症治疗的基石,通过直接的DNA损伤和间接的血管效应起作用.
- 辐射对瘤周细胞的确切影响对于血管稳定至关重要,仍然在很大程度上没有特征.
- 了解辐射对皮细胞的影响对于优化治疗策略至关重要.
研究的目的:
- 为了研究辐射对细胞分化和成熟的影响.
- 阐明辐射影响皮质细胞的潜在分子机制.
- 为了确定辐射是否诱导细胞到肌纤维细胞的转换,在体外和体内.
主要方法:
- 照射小鼠胚胎介质细胞前体细胞 (10T1/2) 和人类胎盘细胞前体细胞 (hPC-PL).
- 使用流细胞计,qRT-PCR和免疫光检测评估细胞体分化,成熟和肌纤维细胞转变.
- 对信号通路 (Akt,ROS) 和体内瘤模型 (NG2DsRed小鼠中的MC38) 的分析,以确认辐射诱导的细胞-肌纤维细胞过渡.
主要成果:
- 辐射促进了细胞周围细胞的分化,并在体外增加了肌纤维细胞标记物表达.
- 辐射激活了Akt信号通路,其抑制,以及ROS抑制,抑制了辐射诱导的肌纤维细胞标记物表达.
- 在体内研究证实了瘤中辐射诱导的皮质细胞到肌纤维细胞的过渡,而Akt的抑制减少了维门的表达.
结论:
- 暴露于辐射会促进细胞周细胞的成熟及其转化为肌纤维细胞.
- 阿克特信号通路在调节辐射对细胞周围细胞的影响方面发挥着至关重要的作用.
- 这些发现为辐射诱导的血管变化和潜在的治疗点提供了洞察力.
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