相关实验视频

Updated: Sep 19, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

来自Callicarpa formosana Rolfe Rolfe的叶子中的生物活性二甲

Panpan Gao¹, Yulu Tian¹, Haowen Luo¹

¹State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

|June 18, 2025

概括

这项研究从Callicarpa formosana中分离出了新型的二类化合物,其中一种化合物表现出抗炎作用,另外两种化合物在细胞模型中表现出肝脏保护活性.

科学领域:

自然产品化学自然产品化学
药理学药理学是指药理学的学科.

背景情况:

卡利卡拉帕 (Callicarpa formosana) 是一种生物活性化合物的植物来源.
迪特尔类是一种具有多样化的生物活动的自然产品类.

研究的目的:

为了从Callicarpa formosana树叶中分离和描述diterpenoids.
评估隔离化合物的抗炎和肝脏保护潜力.

主要方法:

使用色谱技术分离化合物.
通过高分辨率电子喷雾电离质谱法 (HR-ESI-MS),核磁共振 (NMR) 和电子循环二元化 (ECD) 光谱法阐明结构.
在体内抗炎试验和体内肝脏保护试验针对乙氨基 (APAP) 诱导的HepG-2细胞中毒性.

主要成果:

分离了六种新的二类 (五种克莱罗丹和一种拉巴丹) 和六种已知的二类.
化合物4, (2R,5R,8S,9R,10S) -2-乙氧科拉维酸15-甲基,表现出抗炎活性.
化合物7, (5S,8S,9R,10S) -kolavic酸,和12,18-基-13-甲基-labda-7,13-diene-15-oic酸,已经证明了肝保护作用.

结论:

在Callicarpa formosana的化学研究中,获得了具有显著生物活性的新型二甲.
已识别的二甲类物质具有开发作为抗炎和肝脏保护剂的潜力.

关键词:

抗炎药物抗炎药物抗炎药物卡利卡拉帕的形式Rolfe的Rolfe.克莱罗丹二甲化物克莱罗丹二甲化物肝脏保护性肝脏保护性隔离和识别方式

更多相关视频

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Published on: June 27, 2025

相关概念视频

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.