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核向的红色发射碳点用于 in vitro 视觉检测白氨基酶

Shanshan Wei¹, Chengzhao Wang², Kaixin Hu²

¹School of Chemistry and Materials Science, Weinan Normal University, The Western Part of the Middle Section of Chaoyang Street, Weinan 714099, PR China.

International journal of biological macromolecules

|June 18, 2025

概括

一种使用红色发射性碳点 (R-L-CDs) 的新型双模式传感器,可以灵敏和选择性地检测白氨酸氨基酶 (LAP). 这一进步有助于早期诊断药物诱导性肝损伤 (DILI) 和监测肝脏健康.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
纳米技术纳米技术

背景情况:

氨氨基酶 (LAP) 是早期药物诱导性肝损伤 (DILI) 诊断的关键生物标志物.
现有的检测方法缺乏有效临床监测所需的灵敏度和选择性.

研究的目的:

开发一个核准的双模式传感器,用于敏感和选择性的LAP活动检测.
建立一种方法来监测活细胞中的LAP,并了解DILI机制.

主要方法:

使用发射红色的奇拉碳点 (R-L-CD) 作为核心传感材料.
采用基于LAP催化L-氨酸p-nitroaniline的水解的光火机制.
集成色度检测用于基于智能手机的量化.

主要成果:

达到了LAP活动的低检测极限 (0.17U/L用于光,0.69U/L用于色度测量).
在活细胞中证明了LAP的特定检测.
成功验证了乙氨基 (APAP) 诱导的肝毒性产生和修复途径.

结论:

R-L-CD为LAP活动检测提供了一个敏感和选择性的双模式平台.
这种传感器为DILI诊断和临床监测提供了有效的方法.
智能手机兼容的色度检测可方便快速,即时的治疗应用.

关键词:

石化碳点是指石化碳点.双模式传感器传感器莱氏氨基氨基酸酶 (leucine aminopeptidase) 是一种氨基酸酶.

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