酸盐:铁素氧化还原酶的氧化还原场景揭示了经常保留的Fe-S集群潜力
Sheila C Bonitatibus1, Ryan V Pham1, Andrew C Weitz1
1Department of Chemistry, Boston University, Boston, Massachusetts, United States.
The Journal of biological chemistry
|June 18, 2025
概括
铁酸盐:铁酸氧降解酶 (PFOR) 在新陈代谢中起着双重作用. 这项研究使用电化学准确测量PFORs的氧化还原潜力,澄清它们在电子转移中的功能.
科学领域:
- 生物化学 生物化学
- 生物能源学 生物能源学
- 酶学 是一种酶学.
背景情况:
- 铁酸盐:铁酸氧降解酶 (PFORs) 是无氧代谢中的关键酶.
- PFORs既参与二氧化碳的减少和酸盐的合成,也参与产生减少的等效物.
研究的目的:
- 为了研究PFOR中内部电子转移的热力学驱动力.
- 通过使用直接蛋白膜电化学,对PFOR中铁硫集群的氧化还原潜力进行修订后的评估.
主要方法:
- 从四种不同的生物体 (Chlorobaculum tepidum,Magnetococcus marinus,Methanosarcina acetivorans,Desulfovibrio africanus) 获得的PFOR酶的比较分析.
- 直接使用蛋白膜电化学来确定氧化还原潜力.
- 对复合PorD子单元的研究 甲沙辛酸酸 PFOR.
主要成果:
- 确定了四种PFOR酶中铁硫的新,准确的氧化还原潜力.
- 修订后的氧化还原潜力被映射到特定的铁硫集群中,其中包括一种用于Metanosarcina acetivorans PFOR的模型.
结论:
- 这项研究提供了对PFOR中电子转移机制的更精确的了解.
- 准确的氧化还原潜力对于阐明PFORs在无氧能量代谢中的双重氧化和还原作用至关重要.
更多相关视频
12:08Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
15.3K
10:01Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
12.3K
相关概念视频
Ladder Diagrams: Redox Equilibria
537
Ladder diagrams are useful tools for understanding redox equilibrium reactions, especially the effects of concentration changes on the electrochemical potential of the reaction. The vertical axis in the redox ladder diagrams represents the electrochemical potential, E. The area of predominance is demarcated using the Nernst equation.
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
537
Redox Equilibria: Overview
1.1K
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
1.1K
Redox Reactions
219
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
219
Pyruvate Oxidation
161.9K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
161.9K
Role of Reduced Coenzymes NADH and FADH₂
12.7K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
12.7K
Electron Transport Chain: Complex I and II
15.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
15.1K
