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Updated: Sep 19, 2025

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
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使用微分动态显微镜进行分子扩散系数的内流测量

U Eberhard1,2, M Usuelli3, E Secchi1

  • 1ETH Zurich, Department of Civil, Environmental and Geomatic Engineering, 8093 Zurich, Switzerland.

Physical review. E
|June 19, 2025
PubMed
概括

这项研究引入了一种新的显微镜技术,用于测量流动流体中的分子扩散,克服样本变形和不同剪速带来的挑战,以进行准确的运输研究.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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