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Updated: Sep 19, 2025

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在可食用蔬菜作物中的内菌的社区多样性和功能结构的高通量测序分析:对植物微生物质量的潜在影响

Adekunle Raimi¹, Rasheed Adeleke¹

¹Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520 South Africa.

|June 19, 2025

概括

有机农业和传统农业对叶子蔬菜的内菌产生影响. 传统农业增加了细菌的多样性,而植物物种和器官类型显著塑造了微生物群落及其功能.

科学领域:

微生物学微生物学
农业科学农业科学
植物科学植物科学

背景情况:

植物内生细菌居住在植物组织中,影响植物健康和营养循环.
农业实践,包括有机 (OF) 和传统 (CF) 系统,可以改变土壤和植物微生物组.
了解这些微生物群落对于蔬菜的质量和安全至关重要.

研究的目的:

在四种叶菜 (Brassica oleracea,Lactuca sativa,Allium cepa,Spinacia oleracea) 中研究内生菌群的多样性和功能结构.
评估植物物种,器官 (叶子/根) 和养殖系统 (OF与CF) 对这些细菌群落的影响.
预测内菌的功能潜力及其与植物营养成分的相关性.

主要方法:

高通量16S rRNA基因测序用于细菌群落概况.
PICRUSt2管道用于微生物群落的功能预测.
微生物网络分析以识别关键石和枢纽类型.

主要成果:

植物物种和器官类型显著影响了细菌的多样性和组成;洋 (Allium cepa) 群体是不同的.
根表现出比叶子更高的细菌多样性和丰富性.
与有机农业 (OF) 相比,传统农业 (CF) 系统导致总体细菌多样性更高.
确定了诸如Serratia和Streptomyces之类的关键类型.
功能预测揭示了器官特定的代谢能力 (例如,叶子中的碳水化合物降解,根中的营养代谢).
OF农场显示出更高的预测毒性路径,而CF农场显示出更高的生物技术路径.
细菌群落和预测的功能与植物营养素含量有显著的相关性.

结论:

叶菜中的内生细菌群体是由植物物种,器官和农业实践的复杂相互作用形成的.
农业系统对微生物多样性和功能潜力产生重大影响,对蔬菜质量产生影响.
这些发现强调了在农业可持续性和食品安全方面考虑内生菌微生物组的重要性.

关键词:

农业生态系统农业生态系统细菌内细胞是细菌的内细胞.酵素酶是一种酶.肥料肥料的使用方法微生物组是一个微生物组.蔬菜蔬菜是一种蔬菜.

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