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Yan Zhang^1,2, Zhiguo Su³, Xuyang Qiu¹

¹Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment & Ecology, Jiangnan University, Wuxi, 214122, China.

Environmental science and ecotechnology

|June 19, 2025

概括

污水处理厂 (WWTP) 中的1类整合体显示出明显的抗生素耐药性基因 (ARG) 配置文件. 工业WWTP与市政WWTP相比,ARG比例和多样性较低,这表明基因转移动态不同.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

一类整合子是横向基因转移和抗生素耐药性基因 (ARG) 传播的关键驱动因素.
废水处理厂 (WWTP) 是ARG和integrons的重要储库.
了解不同WWTP类型的整体介导的ARG动态至关重要,但有限.

研究的目的:

调查和比较市政和工业WWTP中的整合介导ARG配置文件.
阐明WWTP类型在塑造整合子相关基因转移中的作用.
开发和应用用于分析环境整合的新方法.

主要方法:

使用一种新的方法,结合了嵌套式高通量定量PCR (qPCR) 和PacBio测序.
在市政和工业WWTP样本中分析了1类整子丰度和相关的编码序列.
量化ARG比例和整体内的多样性.

主要成果:

工业WWTP表现出较高的绝对整体丰度,但相对于市政WWTP相比,相对ARG含量较低.
56.8%的检测到的整体子编码序列编码了抗生素耐药性.
工业WWTP整合表明ARG比例较低,多样性减少,非ARG序列的整合增加,表明适应复杂的废水.

结论:

WWTP类型显著影响整合子介导的ARG配置文件和基因转移动态.
工业WWTP整合器似乎整合了更广泛的外源基因,反映了适应特定废水组成的情况.
这项研究为环境整合分析提供了强有力的策略,并增强了对WWTP中ARG传播的理解.

关键词:

抗生素耐药性基因 (ARG) 是指抗生素耐药性的基因.第1类的整合子高通量 qPCRR 的使用.这是PacBio测序.废水处理厂 (WWTPs) 是一个废水处理厂.

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