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脑转移衍生的细胞外囊的多模式成像使用超偏磁铁氧化物纳米粒子标记

Birgitte Feginn Berle¹, Sunniva Juliussen¹, Áurea Castilho¹

¹Department of Biomedicine, University of Bergen, Bergen, Norway.

International journal of nanomedicine

|June 19, 2025

概括

研究人员开发了一种方法,用纳米粒子标记脑转移细胞外囊泡 (EVs),用于MRI跟踪. 这种技术允许体内可视化和跟踪这些关键的转移媒介.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
在瘤学瘤学.

背景情况:

细胞外囊泡 (EVs) 在大脑转移 (BM) 中发挥关键作用,通过形成转移前的.
在体内追踪EVs的分布和相互作用是癌症研究中的一个重大挑战.

研究的目的:

开发和验证一种方法来标记BM-derived EVs使用超偏磁氧化铁纳米粒子 (SPIONs).
通过使用磁共振成像 (MRI) 在体内可视化和跟踪标记EVs.

主要方法:

评估了三种SPION变体,用于标记来自患者的BM细胞系.
使用TEM,铁检测,DLS和NTA进行标记EV的特征.
在胎儿老鼠大脑器官 (FRBOs) 和体内小鼠模型中使用MRI和普鲁士蓝色染色评估功能和可视化.

主要成果:

未涂层的5nmSPION在24小时内实现了>90%的细胞标记效率,而不会影响细胞生长.
间被纳入EV,保持它们的尺寸,并通过MRI实现可视化.
在体内MRI检测证实,在肌肉内注射后,标记EVs的成功跟踪.

结论:

建立了基于SPION的BM衍生电动汽车标签的可靠协议.
开发的方法允许从亚细胞到组织水平的EVs可视化.
这种工具有助于在体内空间跟踪BM-EV,并研究它们在转移中的作用.

关键词:

大脑转移是大脑转移.细胞外囊泡中的细胞外囊泡.磁共振成像技术的使用在前转移前的利基.超超磁性氧化铁颗粒传输电子显微镜的使用

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