在Deuterostomes中EGF-CFC基因的基因组演化
Natalia A Shylo1, Paul A Trainor1,2
1Stowers Institute for Medical Research, Kansas City, Missouri, USA.
概括
EGF-CFC基因家族从一个单一的祖先基因进化,多样化和专注于功能,如脊椎动物的左右图案. 这项研究追踪了它们的进化历史和功能专业化,跨越了deuterostomes.
科学领域:
- 发展生物学 发展生物学
- 进化生物学 进化生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- EGF-CFC蛋白质是脊椎动物胃流动和左右模式的节点信号的关键.
- 在EGF-CFC家族中存在功能专业化,例如,老鼠Cripto (消化) 与CFC1 (左向右模式).
- 限制序列保存使EGF-CFC家族成员的进化和功能分析复杂化.
研究的目的:
- 为了研究EGF-CFC蛋白家族在deuterostomes中的进化史.
- 了解这个家族内的功能专业化和基因重复/损失事件.
- 在像老鼠,斑马鱼和Xenopus这样的模型生物中追踪特定EGF-CFC基因的血统.
主要方法:
- 在deuterostomes跨越EGF-CFC家族基因的家族遗传学分析.
- 比较基因组学来追踪基因进化,扩张和损失.
- 在各种物种中识别祖先基因起源和后代基因.
主要成果:
- EGF-CFC基因家族起源于德乌特罗组祖先的单个基因.
- 基因扩张和功能专业化发生在四足动物中.
- 随后的基因丢失和染色体转位事件在eutherian哺乳动物中被观察到.
- 像老鼠Cripto,CFC1,斑马鱼Tdgf1,和Xenopus Tdgf1,Tdgf1.2,Cripto.3这样的特定基因是祖先Tdgf1.1.3的后裔.
结论:
- 祖先的双胞胎体Tdgf1基因多样化为多个家族成员.
- 克塞诺普斯Tdgf1.2 (Tdgf1B) 专注于左向右的图案设计.
- 哺乳动物CFC1在染色体转位后保留了这种左右模式专业化.
更多相关视频
09:31Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Published on: January 12, 2015
11.2K
07:34The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
Published on: February 16, 2017
8.1K
相关概念视频
Cis-regulatory Sequences
10.2K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.2K
Exon Recombination
3.7K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.7K
Gene Duplication and Divergence
6.4K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.4K
Gene Evolution - Fast or Slow?
7.5K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.5K
Eukaryotic Evolution
36.8K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
36.8K
Genome Size and the Evolution of New Genes
8.4K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
8.4K
