使用pET01载体进行小基因拼接试验的协议

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Updated: Sep 19, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

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Hannah Andreae1, Marialessandra Curcio2, Daniel Owrang1

  • 1Institute of Human Genetics, University Medical Center Göttingen, 37073 Göttingen, Germany; Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37073 Göttingen, Germany; Auditory Neuroscience and Optogenetics Laboratory, German Primate Center, 37077 Göttingen, Germany.

STAR protocols
|June 19, 2025
PubMed
概括

这项研究引入了一种小基因拼接试验协议,用于分析遗传性疾病中异常拼接. 该方法对于在没有患者样本的情况下研究拼接变体是有价值的.

关键词:
基于细胞的测试遗传学 是一个遗传学.基因组学就是基因组学.分子生物学分子生物学测序测序是指测序的时间.

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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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