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通过酸的暗发酵增强生物生产:对微生物组成和功能基因的洞察

Liguo Zhang^1,2, Yanan Bai¹, Jing Sang¹

¹College of Environmental and Resource Sciences, Shanxi University, Taiyuan 030006, P.R. China.

Journal of microbiology and biotechnology

|June 19, 2025

概括

酸 (HA) 从葡萄糖中显著增加了26.6%以上的生物生产. 这项研究探讨了HA如何影响微生物群落和功能基因,以改善可持续的能发电.

科学领域:

生物技术是生物技术.
环境科学环境科学
微生物学微生物学

背景情况:

从有机废物生产生物是一种关键的环保能源战略.
目前的生物产量有限,需要增强方法.
酸 (HA) 被探索为固体氧化还原媒介,以提高产量.

研究的目的:

通过使用酸 (HA) 来研究从葡萄糖中增强生物生产的方法.
阐明涉及微生物社区转移和功能基因表达的潜在机制.
为了确定最佳的HA剂量以最大限度地提高生物产量.

主要方法:

葡萄糖与不同度的湿酸 (HA) 的发酵.
挥发性脂肪酸 (VFA) 和副产品度的分析.
使用16S rRNA测序进行微生物社区分析.
冗余分析 (RDA) 用于将微生物组成与生物产量相关联.
功能基因的KEGG通路分析.

主要成果:

80和150毫克/升的最佳HA剂量分别产生了312.7和315.5毫升/克的葡萄糖,比对照组增加了>26.6%.
乙酸度在80mg/l HA时显著增加.
细菌属*Thermomarinilinea*的相对丰度增加了1.0到3.9倍.
生物产与包括*Gimesia*,*Longilinea*,*Defluviimonas*,*Pirellula*和*Planctomicrobium*在内的属相关.
关键的生物相关基因没有显示显著的上调,这表明HA通过电子转移和微生物社区调节来增强产量.

结论:

胺酸有效地增强了从葡萄糖中产生生物的产量.
该机制涉及加速电子转移和调节微生物社区结构,而不是直接调节特定基因.
这项研究提供了对优化生物生产的见解,使用可再生能源的氧化还原媒介来优化生物生产.

关键词:

胺酸是一种胺酸.生物的生物.功能性基因是指功能性基因.运动学的动力学.微生物社区的微生物社区.

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