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EIF4A3诱导的Circ_0092278通过PI3K/Akt/mTOR信号通路增强了乳头甲状腺癌细胞恶性病变

Mengjiang Liu¹, Linghui Zhang¹, Zhaodan Yan¹

¹Department of Endocrinology, Hubei No. 3 People's Hospital of Jianghan University, Wuhan 430033, Hubei, P.R. China.

Journal of microbiology and biotechnology

|June 19, 2025

概括

循环RNAcirc_0092278通过激活PI3K/AKT/mTOR通路来促进乳头甲状腺癌 (PTC) 的进展. 针对EIF4A3-circ_0092278相互作用提供了一个潜在的新治疗策略,用于PTC.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
生物化学生物化学

背景情况:

循环RNAs (circRNAs) 与癌症有关,但circ_0092278在乳头甲状腺癌 (PTC) 中的特定作用仍然基本上是未知的.
了解circRNA调节对于开发新型癌症疗法至关重要.

研究的目的:

为了研究circ_0092278在PTC进展中的功能作用.
探索circ_0092278和真核细胞翻译启动因子4A3 (EIF4A3) 之间的相互作用.
阐明潜在的分子机制,包括PI3K/AKT/mTOR途径.

主要方法:

定量实时聚合酶连锁反应 (qRT-PCR) 用于表达分析.
在体外细胞实验中评估增殖,迁移和入侵.
西部涂抹检查PI3K/AKT/mTOR通路蛋白质.
在体内异种移植小鼠模型用于瘤生长评估.
检测RNA结合蛋白免疫沉降 (RIP) 试验以确认分子相互作用.

主要成果:

Circ_0092278在PTC组织中显著上调,位于细胞质中.
通过抑制PI3K/AKT/mTOR通路来抑制circ_0092278抑制了PTC细胞的增殖,迁移,入侵和瘤生长.
通过PI3K/AKT/mTOR通路激活,circ_0092278的过度表达增强了PTC细胞恶性瘤.
发现EIF4A3与MINK1结合,增强circ_0092278的表达,建立了一个EIF4A3-circ_0092278调节轴.

结论:

Circ_0092278在PTC中充当瘤性circRNA,促进瘤进展和恶性病变.
EIF4A3-circ_0092278轴通过激活PI3K/AKT/mTOR路径来推动PTC的发展.
针对EIF4A3-circ_0092278相互作用,为乳头甲状腺癌提供了一个有希望的治疗策略.

关键词:

欧洲投资基金4A3A3 在PI3K/Akt/mTOR中使用.环_009227878 的时间表.皮囊性甲状腺癌是什么?

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