相关实验视频

Updated: Sep 18, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

RNA测序揭示了慢性间歇性缺氧中循环RNA表达模式

Cong Li¹, Tiantian Su¹, Yuqin Chen¹

¹Department of Otorhinolaryngology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai, 200336 China.

Sleep and biological rhythms

|June 20, 2025

概括

这项研究揭示了暴露于慢性间歇性缺氧 (CIH) 的老鼠中改变的循环RNA (circRNA) 表达,这种情况模仿阻塞性睡眠呼吸暂停 (OSA). 这些发现表明了诊断CIH和OSA的潜在生物标志物.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
睡眠医学睡眠医学

背景情况:

循环RNAs (circRNAs) 在生物过程中至关重要,但它们在阻塞性睡眠呼吸暂停 (OSA) 引起的慢性间歇性缺氧 (CIH) 中的作用尚不清楚.
在CIH下研究circRNA表达模式对于理解OSA病理生理学至关重要.

研究的目的:

在CIH的反应中识别差异表达的circRNAs (DEcircRNAs).
在OSA患者中验证DEcircRNAs的诊断潜力.
探索CIH中的circRNA-miRNA调节网络.

主要方法:

在CIH大鼠模型和诺莫西克对照中进行了RNA测序.
差异表达的circRNAs被确定并过.
候选circRNAs在OSA患者和使用qRT-PCR的对照中得到了验证.
主要组件分析 (PCA) 评估了诊断潜力.
miRanda软件预测了目标miRNA,而Cytoscape则可视化了监管网络.

主要成果:

确定了43个DEcircRNAs,富含与蛋白质结合和细胞骨组织相关的功能.
在8个验证的circRNA中,有5个显示CIH和对照组之间的显著表达差异.
这些circRNAs表现出明显的表达模式,表明CIH诊断的潜力.
建立了一个circRNA-miRNA调节网络,突出了mir-466b-3p与CIH的关联.

结论:

这项研究在CIH条件下描述了circRNA表达特征.
确定了几种表达变化的circRNA,表明它们参与CIH.
这些发现为进一步研究circRNA功能和在OSA和CIH中作为生物标志物的潜力提供了基础.

关键词:

慢性间歇性缺氧慢性间歇性缺氧循环RNAs是一种循环RNA.微RNAs是一种微RNA.阻塞性睡眠呼吸暂停症是什么在RNA测序过程中,RNA测序

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