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相关概念视频

Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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Updated: Sep 18, 2025

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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通过代谢编程,LKB1调节ILC3的产后发育和效应器功能.

Huasheng Zhang1,2,3, Linfeng Zhao1,2,3, Qingbing Zhang1,2,3

  • 1Center for Immune-Related Diseases at Shanghai Institute of Immunology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Frontiers in immunology
|June 20, 2025
PubMed
概括
此摘要是机器生成的。

肝激酶B1 (LKB1) 对第3组先天性淋巴细胞 (ILC3) 的发展和功能至关重要,这些细胞对肠道免疫至关重要. 缺乏LKB1会损害ILC3s,影响肠道平衡和宿主防御.

关键词:
肝激酶B1 (LKB1) 的使用第三组先天性淋巴细胞 (ILC3s)这是一种炎症炎症炎症炎症.肠道免疫常态稳定.代谢编程是一种代谢编程.

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相关实验视频

Last Updated: Sep 18, 2025

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科学领域:

  • 免疫学 免疫学 免疫学
  • 代谢过程中的代谢.
  • 细胞生物学 细胞生物学

背景情况:

  • 第三组先天性淋巴细胞 (ILC3s) 对于维持肠道平衡和宿主防御至关重要.
  • 代谢调节显著影响ILC3的激活和功能.
  • 肝激酶B1 (LKB1) 是一种关键的代谢调节剂,其在ILC3功能和肠道免疫力中的特定作用尚不清楚.

研究的目的:

  • 研究LKB1在肠道ILC3s中的作用.
  • 确定LKB1如何影响ILC3的发育,功能和新陈代谢.
  • 阐明LKB1对肠道免疫和宿主防御的贡献.

主要方法:

  • 使用Rorc和Stk11菌株生成了LKB1条件淘汰赛小鼠.
  • 通过流式细胞计量评估ILC3细胞数量和细胞因子生产.
  • 利用Citrobacter rodentium感染模型和RT-qPCR,流细胞计和免疫组织化学来评估肠道防御和炎症反应.

主要成果:

  • LKB1对于ILC3的产后发育至关重要,而LKB1缺乏的小鼠显示ILC3数量减少.
  • 在ILC3s中切除LKB1减少了IL-22的产生,并损害了对Citrobacter rodentium感染的保护.
  • 缺乏LKB1会影响ILC3的新陈代谢,由降低的糖解,氧化酸化和线粒体质量证明.

结论:

  • LKB1是肠道ILC3发育和效应器功能的关键调节者.
  • LKB1将新陈代谢控制与肠道免疫平衡联系起来.
  • 这些发现强调了LKB1在维持肠道健康方面的重要性,并提出了潜在的治疗途径.