甲素附加的Mn-MOF对人类血清白蛋白及其细胞影响的结构影响
Shanmugavel Chinnathambi1, Mahima Kumar1,2, Basudeb Dutta3
1Institute for Integrated Cell-Material Sciences, Institute for Advanced Study, Kyoto University, Kyoto 606-8501, Japan.
ACS applied bio materials
|June 20, 2025
概括
绿色发射的基金属有机框架 (Mn-MOF) 纳米颗粒对人血清白蛋白具有很高的亲和力,保留了它的结构. 这些生物相容的Mn-MOF对活细胞成像和纳米医学应用具有前景.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 金属有机框架 (MOF) 为生物医学用途提供可调节的特性.
- 基于的MOFs (Mn-MOFs) 正在探索其成像潜力.
研究的目的:
- 合成绿色发射,水可分散的Mn-MOF纳米粒子.
- 评估Mn-MOF与人血清白蛋白 (HSA) 的相互作用.
- 评估Mn-MOF生物相容性和细胞吸收,用于活细胞成像.
主要方法:
- 对HSA相互作用进行光谱分析 (光,圆形二重化).
- 对HeLa,A549和冠状细胞细胞系的细胞毒性测定.
- 对焦显微镜和流细胞计用于细胞吸收研究.
主要成果:
- Mn-MOFs对HSA表现出高亲和度的结合,保持其原生形状.
- 在中度度下,纳米粒子表现出良好的生物相容性和高细胞活力.
- 通过内细胞分裂观察到有效的细胞吸收,具有周核局部化.
- 在较高度下,观察到剂量依赖性毒性,影响细胞粘附和活力.
结论:
- Mn-MOF纳米粒子在结构上与像HSA这样的蛋白质兼容.
- 这些Mn-MOF表现出低细胞毒性和高效的细胞吸收,适合活细胞成像.
- Mn-MOFs为纳米医学提供了一个可行的平台,包括有针对性的输送.
相关概念视频
01:20Factors Affecting Protein-Drug Binding: Protein-Related Factors
280
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
280
01:29Factors Affecting Protein-Drug Binding: Patient-Related Factors
123
Protein-drug binding, a pivotal aspect of pharmacokinetics, is subject to considerable variability influenced by an array of patient-related factors. The intricate interplay of age, individual differences, and pathological conditions significantly impact the binding dynamics and subsequent pharmacological effects.
Age stands as a key determinant in protein-drug binding. Neonates, characterized by low albumin content, experience heightened concentrations of unbound drugs such as phenytoin and...
Age stands as a key determinant in protein-drug binding. Neonates, characterized by low albumin content, experience heightened concentrations of unbound drugs such as phenytoin and...
123