定数感应通过调节二次代谢产物来调节微生物社区结构
April C Armes1, Amy L Schaefer2, Leah H Hochanadel1
1Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
mSphere
|June 20, 2025
概括
在一个合成细菌群体中破坏乙烯基荷莫塞林乳 (AHL) 质量感应 (QS) 改变了主导成员的丰富性和代谢概况,但不是整体社区成员. 这突出了QS.
科学领域:
- 微生物生态学 微生物生态学
- 细菌社区的动力学
- 质量检测 (QS) 机制 质量检测的机制
背景情况:
- 微生物之间的相互作用对多种细菌群落至关重要,但它们对社区结构的影响尚不清楚.
- 通过QS进行细胞间通信,调节二次代谢物产生和微生物相互作用.
- 氨基荷莫塞林乳 (AHL) 介导的QS是许多细菌的关键信号通路.
研究的目的:
- 研究AHL介导的QS对合成细菌群体的结构和动态的影响.
- 为了确定破坏QS如何影响微生物之间的相互作用和代谢概况.
- 为了评估微生物社区成员在改变的QS信号下的稳定性.
主要方法:
- 使用一种由*Populus deltoides*衍生而来的10个成员合成细菌群体.
- 使用AiiA-lactonase破坏了AHL信号传输,AiiA-lactonase是一种降解AHLs (定数灭) 的酶.
- 社区结构 (16S rRNA测序) 和二次代谢物产量在连续传递后被评估;双重抑制试验评估了微生物-微生物相互作用.
主要成果:
- AHL无活化改变了后来的通道中占主导地位的社区成员的相对丰富性,但没有影响整个社区成员数量.
- 质量灭显著改变了受治疗群体的代谢概况.
- 代谢变化导致其他社区成员的抑制减少,影响微生物与微生物的相互作用.
结论:
- QS,特别是AHL介导的信号传递,通过调节主导物种中的二次代谢物来影响细菌群体结构.
- 微生物社区的成员可以保持稳定,尽管代谢活动和微生物之间的相互作用发生了重大变化.
- 了解QS机制对于预测和操纵微生物生态系统生态学至关重要,在农业和可持续系统中有应用.
相关概念视频
Gene Regulation in Microbial Communities: Quorum Sensing
97
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
97
Bacterial Signaling
34.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
34.6K
Global Regulatory Systems
83
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
83
Translational Regulation
110
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
110
Gene Regulation During Sporulation
85
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
85
Stringent Response in E. coli
61
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
61


