马中的内分泌特征和OPU-ICSI结果:一项比较研究
Dragos Scarlet1,2,3, Gerhard Schuler4, Eleni Malama2,3
1Institute of Veterinary Anatomy, Vetsuisse Faculty Zurich, Zurich, Switzerland.
Reproduction & fertility
|June 20, 2025
概括
高水平的孕激素 (P4) 可能会对马的卵子采集 (OPU) 和细胞内精子注射 (ICSI) 的成功产生负面影响. 了解马匹激素的特征,包括抗穆勒尔激素 (AMH),可以帮助预测OPU-ICSI的结果.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
背景情况:
- 马类卵子采集 (OPU) 和细胞内精子注射 (ICSI) 的成功率显示出显著的个体变化.
- 影响马匹OPU-ICSI结果的荷尔蒙因素尚未完全理解.
研究的目的:
- 调查马匹血清激素度与OPU-ICSI程序的成功之间的相关性.
- 为了确定与低或高胚胎产量相关的特定激素配置文件.
主要方法:
- 在OPU时,测量了59只母马的血清激素水平 (GDF9,BMP15,AMH,P4,E2).
- 马被追溯分为低 (<2个胚胎) 或高 (≥2或≥3个胚胎) 结果组.
- 荷尔蒙水平与胚胎产量和其他OPU参数进行了分析.
主要成果:
- 母的年龄,BCS,周期阶段或卵细胞恢复率都没有影响OPU-ICSI结果.
- 每次产生≥3个胚胎的母马的P4水平较低,AMH水平较高 (P<0.05).
- 获得≥2个胚胎的概率>90%与特定的P4和E2度有关.
结论:
- 高水平的孕激素可能与马匹的OPU-ICSI结果有负相关.
- AMH和类固醇激素 (P4,E2) 概况显示出作为OPU-ICSI成功的预测因素的潜力.
- 需要进一步的研究来阐明复杂的荷尔蒙对马类胚胎生产的影响.
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