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Updated: May 11, 2026

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
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Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

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FRAP4ICBS:FRAP分析方法用于评估生物分子凝结物形成的主导效应

Xiaotian Wang1,2, Zhiguang Xiao1,2, Jiahao Niu1,2

  • 1National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China.

Analytical chemistry
|June 20, 2025
PubMed
概括

一种名为FRAP4ICBS的新方法区分了凝结物形成机制,确定了与空间聚类结合位点 (ICBS) 的相互作用在DNA/RNA依赖的凝结物中占主导地位,挑战了先前的液态-液态相分离 (LLPS) 假设.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...