相关实验视频
Updated: Sep 18, 2025

09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
14.7K
在g-tubulin-FTO-MARK4翻译微域上合成的超急性反应蛋白调节癌症的急性应激反应
Bruno Saleme1, Saymon Tejay1, Kléouforo-Paul Dembélé1
1Department of Medicine, University of Alberta, Edmonton, AB T6G 2G5, Canada.
Cell reports
|June 20, 2025
概括
癌细胞通过专门的翻译机制迅速产生应激反应蛋白 (HARP). 针对这些途径,包括FTO和MARK4,可以克服癌症.
科学领域:
- 细胞应激反应的细胞应激反应
- 癌症生物学 癌症生物学
- 翻译的分子机制.
背景情况:
- 与正常细胞相比,癌细胞表现出更强的抗压能力.
- 在癌症中,应激反应对于适应和治疗耐药性至关重要.
- 全球翻译通常在细胞压力下被抑制.
研究的目的:
- 为了识别癌细胞在压力下快速翻译的蛋白质.
- 阐明控制这种快速蛋白质合成的分子机制.
- 探索克服癌症抗压力的潜在治疗目标.
主要方法:
- 无偏的蛋白质组学和转录组学,以识别超急性反应蛋白 (HARP).
- 研究mRNA翻译部位,特别是微管相关的翻译微域 (MATM).
- 分析FTO (RNA脱甲基酶) 和MARK4 (激酶) 在HARP调节中的作用.
主要成果:
- 在20分钟内从先前存在的mRNA中翻译出已识别的HARP.各种压力.
- 发现的HARP mRNA翻译发生在MATM上的g-tubulin上,涉及FTO和特定的核糖体.
- 由MARK4激活的FTO去甲基化了抑制的m6A标记,使分隔的HARP转化成为可能.
- 在压力下抑制FTO或MARK4抑制了HARP合成并增加了癌细胞亡.
结论:
- 哈普翻译代表了癌症中一种新的应激适应机制.
- γ-蛋白-FTO-MARK4轴对于HARP合成至关重要.
- 针对这一轴提供了一个潜在的策略,使癌细胞对压力和化疗敏感.
相关概念视频
Other Stress Responses in Bacteria
70
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
70
Regulation of the Unfolded Protein Response
2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
Stringent Response in E. coli
61
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
61
mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
Microtubule Instability
5.3K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.3K
Destabilization of Microtubules
2.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.8K

