生物无活性白蛋白表面能够实现超高的血管细胞选择性,优于特定的结合性连接体
Yifeng Chen1,2, Hongye Hao1,2,3, Yijing Yin2
1State Key Laboratory of Transvascular Implantation Devices, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, P. R. China.
ACS nano
|June 20, 2025
概括
专涂层显著增强了内皮细胞对材料表面光滑肌肉细胞的选择性,改善了心血管设备的性能. 这种新的方法通过促进内皮质化来预防复原和血栓形成.
科学领域:
- 生物材料科学 生物材料科学
- 心血管研究研究心血管研究
- 表面化学 表面化学
背景情况:
- 改善心血管设备的结果需要增强内皮细胞 (EC) 选择性,而不是植入物表面上的光滑肌细胞 (SMC).
- 目前使用特定配体的表面修饰方法产生较低的EC/SMC比率 (<10),限制了有效性.
- 静脉复原和晚期血栓形成仍然是心血管设备植入的重大挑战.
研究的目的:
- 调查白蛋白涂层在材料表面上实现高EC选择性的潜力.
- 探索白蛋白介导的EC/SMC选择性背后的机制.
- 在动物模型中评估蛋白修饰表面的体内性能.
主要方法:
- 材料的表面修饰用白蛋白进行.
- 在实验室中使用内皮细胞和光滑肌细胞在血清补充介质中进行细胞粘附测定.
- 机制研究以阐明细胞粘附调节的模式.
- 在动物模型中进行体内评估,以评估内增生和内皮细胞化.
主要成果:
- 专涂层实现了前所未有的EC/SMC比率超过200,显著优于现有方法.
- 选择性主要是由SMC粘附的阻抗驱动的,而不是增强的EC粘附.
- 在体内研究表明,成功抑制亲密增生和促进植入体内皮质化.
结论:
- 简单的白蛋白修饰提供了一种高度有效的策略,用于增强心血管器件表面的EC选择性.
- 这种方法具有显著的潜力,可以改善可植入心血管器械的临床性能.
- 专涂层促进了完整的内皮质化,减轻了复原和血栓形成的风险.
相关概念视频
Drug Distribution: Plasma Protein Binding
6.8K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
6.8K
Factors Affecting Protein-Drug Binding: Protein-Related Factors
280
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
280
Drug Binding to Blood Components
272
When drugs enter systemic circulation, they interact with various components of the blood, including proteins such as human serum albumin (HSA), α1-acid glycoprotein (AAG), lipoproteins, globulins, and red blood cells (RBCs).
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...
272
Drug Distribution: Tissue Binding
3.2K
Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...
For...
3.2K


