在Synechocystis sp.中从数据库中有效地选择pyruvate脱碳酶序列,以提高乙醇生产率. 在PCC 6803中
Hiroki Nishiguchi1, Teppei Niide1, Yoshihiro Toya1
1Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
Journal of bioscience and bioengineering
|June 20, 2025
概括
这项研究确定了适合的细菌酸盐脱碳酶 (PDCs),用于在蓝藻细菌中增强乙醇生产. 工程和自然PDC扩大了生物燃料应用中可用的序列的范围.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 像Synechocystis sp.这样的蓝藻细菌 PCC 6803是可持续乙醇生产的关键目标.
- 在这些生物体中,酸盐脱碳酶 (PDC) 的异质表达对于将酸盐转化为乙醇至关重要.
- 缺乏系统地寻找最佳的PDC酶.
研究的目的:
- 识别和评估天然和人工的酸盐脱碳酶 (PDCs),以在Synechocystis sp.中有效生产乙醇. 这是PCC 6803.
- 扩大用于生物燃料应用的功能性PDC的目录.
- 建立一个强大的酶发现和工程战略.
主要方法:
- 使用两步选方法来评估用于乙醇生产的9个天然PDC.
- 来自Zymomonas mobilis和Gluconobacter diazotrophicus的细菌PDC显示出活动.
- 进一步的评估包括来自各种细菌来源的自然和人工PDC (祖先序列重建,设计序列).
主要成果:
- 使用来自Zymomonas mobilis和Gluconobacter diazotrophicus的PDCs确认了乙醇的生产.
- 与Zm PDC相比,来自Gluconobacter oxydans的PDC显示出更高的乙醇生产率 (88.9 mg/L/5天).
- 通过以前未经证实和工程PDCs实现了乙醇生产,扩大了适用的酶的范围.
结论:
- 细菌PDC适合在蓝藻细菌中进行异质表达和乙醇生产.
- 一个逐步的策略有效地识别和设计酶,以改善生物燃料合成.
- 这项研究扩大了可用于蓝藻细菌乙醇生产的PDC序列的范围.
相关概念视频
01:20Fates of Pyruvate
9.0K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
9.0K
01:15Pyruvate Oxidation
161.9K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
161.9K