在DIVARICATA1通过调节花期整合剂和Arabidopsis中的GA生物合成促进了开花
Dong Li1, Xumin Wang2, Tiantian Liu3
1The Engineering Research Institute of Agriculture and Forestry, College of Horticulture, Yantai Technology Center of Characteristic Plant Gene Editing & Germplasm Innovation, Ludong University, Yantai 264025, Shandong Province, China.
International journal of biological macromolecules
|June 20, 2025
概括
阿拉比多普西斯的转录因子AtDIV1通过调节GA信号来促进开花. 过度表达会加速开花,而突变会延迟开花,突出了AtDIV1的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 遗传学 是一个
背景情况:
- 已知阿拉比多普西斯R-R型MYB类转录因子AtDIV1和AtDIV2 (DIVARICATAs) 参与盐度应激反应.
- 在调节开花过渡过程中AtDIV1的作用仍然未被探索.
研究的目的:
- 研究阿拉比多普西斯转录因子AtDIV1在调节开花过渡过程中的作用.
- 阐明AtDIV1介导的开花调节背后的分子机制.
主要方法:
- 蛋白质序列和3D结构的比较分析.
- 在不同发育阶段对AtDIV1表达模式的分析.
- 产生和分析AtDIV1过度表达和淘汰突变.
- 转录组分析以确定受调节的基因.
- 染色体免疫沉 (ChIP) 和电泳运动转移试验 (EMSA) 来确定直接的DNA结合.
- 用外源GA3和paclobutrazol进行吉伯雷林 (GA) 含量测量和救援实验.
主要成果:
- 在AtDIV1高度保存,并在Arabidopsis.在螺栓化阶段表达.
- 过度表达AtDIV1促进了开花,而AtDIV1突变 (div1-1, div1-2) 推迟了开花.
- AtDIV1直接结合到花期相关基因TEM1,SOC1和GA3ox1.1的促进子区域.
- AtDIV1影响GA生物合成,过度表达增加GA水平,突变降低GA水平.
- 外源性GA3拯救了div1突变的晚期开花表型,而帕克洛布特拉则拯救了AtDIV1过度表达者的早期开花表型.
结论:
- 在Arabidopsis.IV1中,AtDIV1充当了开花过渡的积极调节者.
- 在DIV1中介于通过吉伯雷林 (GA) 信号通路进行开花.
- AtDIV1直接调节关键的开花和GA生物合成基因,将GA信号整合到开花时间控制中.
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