植物野生相对种群的Beta vulgaris作为复杂特征的全基因组关联映射的来源
Lisa Bertram1,2, Friedrich Kopisch-Obuch3, Matthias Frisch4
1Institute of Agronomy and Plant Breeding II, Justus Liebig University, Giessen, Germany. lisa.bertram@kws.com.
概括
野生甜菜种群为检测影响干旱耐受性等特征的小基因提供了有价值的遗传变异. 最佳检测需要将野生甜菜与精英品种交叉,以平衡遗传多样性和准确的表型.
科学领域:
- 遗传学和植物育种 植物育种
- 农业科学 农业科学
背景情况:
- 野生甜菜种群是遗传变异的未充分利用的资源.
- 检测野生甜菜中定量特征的小基因是具有挑战性的.
- 现有的方法无法充分利用野生甜菜的遗传资源.
研究的目的:
- 评估野生甜菜种群属性,特征架构和人口结构映射对定量特征位置 (QTL) 检测的影响.
- 确定最佳的交叉设计,用于在繁殖计划中利用野生甜菜的遗传资源.
- 评估不同测绘种群在检测小基因方面的能力.
主要方法:
- 使用了来自三个野生甜菜种群的基因型数据.
- 模拟了9个交叉设计,以创建映射群体.
- 对于每个设计,都评估了检测小QTL和错误检测率的功率.
主要成果:
- 绘制50%野生甜菜基因组的种群显示了QTL检测的最高功率.
- 低于1%的等位基频率的小QTL可以用合理的功率检测到.
- 在实地试验中,至少需要75%的精英基因组才能有效地表型化.
结论:
- 野生甜菜种群是发现控制复杂特征的基因的宝贵来源.
- 使用精英x野生甜菜F1杂交的交叉设计对全基因组关联映射最有效.
- 优化的入侵策略是利用野生甜菜在作物改进中的遗传多样性的关键.
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