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在不同毛囊发育阶段,水牛卵巢组织中生长因子的表达

Seham Samir Soliman¹, Marwa El-Sheikh², Dalia A Taha³

¹Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre (NRC), Dokki, Cairo, 12622, Egypt. sehamsamir81@yahoo.com.

Archives of gynecology and obstetrics

|June 21, 2025

概括

增长标记基因表达 (BMP15,GDF9,OCT4,FGFR2) 和氧化应激标记因水牛卵巢的毛囊大小而有所不同. 这些发现为改善辅助生殖结果提供了对卵泡发育的见解.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
辅助生殖技术是一种辅助生殖技术.

背景情况:

卵巢反应不良会影响辅助生殖过程中的卵细胞产量.
遗传因素显著影响卵巢反应.

研究的目的:

在卵巢组织中量化BMP15,GDF9,OCT4和FGFR2的mRNA表达.
与特定的毛囊发育阶段相关联基因表达.
评估与毛囊大小相关的氧化应激标志物 (CAT,GPx,SOD,ROS,MDA).

主要方法:

收集了卵巢组织样本.
用RT-qPCR进行基因表达分析.
ELISA测量了蛋白质度和氧化应激标志物.

主要成果:

OCT4,BMP15,GDF9和FGFR2在毛囊大小之间显示出不同的表达模式.
在小毛囊中,SOD活性最高.
随着毛囊大小的增加,GPx活动增加,而ROS,MDA和CAT减少.

结论:

基因表达和氧化应激标记与水牛的毛囊发育阶段有关.
了解这些分子动力学可以提高辅助生殖计划中的生殖效率.

关键词:

布法罗水牛在线观看毛囊的发育毛囊的发展卵巢组织的卵巢组织.氧化应激是一种氧化应激.在mRNA转录过程中,

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