埃克迪斯特对胸细胞体积调节和增殖的双向作用
Fariza A Salimova1, Sarvinoz I Rustamova1, Sirojbek A Khojiboev1
1Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent 100174, Uzbekistan.
埃克迪斯特 (20-hydroxyecdysone,20HE) 影响胸腺细胞,最初阻断体积敏感通道,但在长时间暴露时促进生长和细胞体积调节. 这表明对T细胞发育有复杂的,剂量依赖的影响.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- T细胞的发育和成熟取决于细胞体积调节 (CVR).
- 对体积敏感的向外调整阳离子通道 (VSOR/VRAC) 对CVR至关重要.
- 埃克迪斯特 (20-hydroxyecdysone, 20HE) 对胸腺和胸细胞的影响基本上是未知的.
研究的目的:
- 为了研究20HE对胸腺细胞性和功能的影响.
- 确定20HE对胸细胞体积调节 (CVR) 和相关通道的影响.
- 探索小胞细胞对20HE的短期和长期反应.
主要方法:
- 评估暴露于不同20HE度的胸细胞中的VSOR/VRAC活性和调控体积减少 (RVD).
- 在20HE治疗后,在初级培养中评估胸细胞增殖.
- 在口服20HE后,在小鼠中测量胸膜生长和CVR效率.
主要成果:
- 短期20HE暴露抑制了胸细胞中的VSOR/VRAC活性和RVD.
- 长时间暴露于20HE会增加胸细胞的增殖,并提高心电回路效率.
- 在体内给予20HE刺激了胸膜生长,改善了细胞CVR.
结论:
- 20HE对胸细胞具有双向作用,具有急性抑制和慢性刺激的CVR和增殖.
- 20HE的长期系统性作用可能会克服其对胸细胞VSOR/VRAC和RVD的急性抑制作用.
- 20HE通过复杂调节细胞体积调节和增殖来影响T细胞发育.
更多相关视频
08:56Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
Published on: June 9, 2015
12:10Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
Published on: April 14, 2017
相关概念视频
Functions of Thyroid Hormones
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Target Cell Response to Hormones
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
Regulation of Hormone Secretion
Humoral...
Cells Coordinate Growth and Proliferation
