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超强的RBM特异单域抗体广泛中和了具有皮科莫尔活性的多个SARS-CoV-2变体

Dmitry V Shcheblyakov¹, Irina A Favorskaya¹, Inna V Dolzhikova¹

¹Federal State Budget Institution "National Research Center for Epidemiology and Microbiology Named after Honorary Academician N.F. Gamaleya" of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia.

International journal of biological macromolecules

|June 21, 2025

概括

一个新的单域抗体,1p1B10显示了对包括Omicron亚系在内的众多SARS-CoV-2变体的广泛中和. 这种抗体通过阻止病毒进入细胞,为治疗COVID-19提供了一个有希望的治疗候选者.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
结构生物学结构生物学

背景情况:

SARS-CoV-2 感染会导致严重的疾病,特别是在高风险群体中,抗病毒选择有限.
新兴的SARS-CoV-2变种具有众多突变,对治疗开发构成重大挑战.

研究的目的:

描述一种广泛中和的单域抗体,1p1B10,对各种SARS-CoV-2变体具有强大的活性.
阐明1p1B10.10的广泛中和活动的作用机制.

主要方法:

针对多种SARS-CoV-2变异的1p1B10抗体活性的表征.
高分辨率的晶体结构确定与1p1B10复合的S蛋白受体结合域 (RBD).
在叙利亚仓鼠和 hACE2小鼠的体内疗效研究.

主要成果:

1p1B10表现出对广泛的SARS-CoV-2变体的皮科莫尔活性,包括Omicron亚系 (例如XBB.1,JN.1).
结构分析显示,1p1B10与RBD结合,不受突变影响,并与ACE2受体结合竞争.
治疗用1p1B10-Fc降低了子的病毒载量,并保护小鼠免受致命的感染.

结论:

1p1B10对当前和新兴的SARS-CoV-2变种表现出广泛的中和活性.
抗体的机制包括通过ACE2受体竞争阻止病毒附着.
1p1B10是COVID-19的治疗方法中的一个有前途的候选者.

关键词:

在 COVID-19 疫情中,单克隆抗体是一种单克隆抗体.中和活动活动.中和抗体的抗体单域抗体是一种单域抗体.结构分析结构分析治疗疗效疗效疗效疗效疗效疗效疗效疗效

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