相关实验视频
Updated: Aug 1, 2026

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Zika Virus Specific Diagnostic Epitope Discovery
Published on: December 12, 2017
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一个高亲和力和潜在的中和的纳米体对抗寨卡病毒
Huan Hu1, Qiang Deng2, Cheng Guo3
1Department of Biomedical Sciences, City University of Hong Kong, Hong Kong 518057, China.
ACS infectious diseases
|June 22, 2025
概括
研究人员开发了针对寨卡病毒 (ZIKV) 包膜蛋白域III (EDIII) 的新型纳米体. 特定的纳米体,Nb-E3和Nb-E5,证明了强大的ZIKV中和,为抗击寨卡病毒感染提供了潜在的新治疗策略.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 寨卡病毒 (ZIKV) 是一种新兴的病原体,目前没有疫苗或治疗方法.
- ZIKV包膜蛋白域III (EDIII) 诱导中和抗体,但以前没有针对它的纳米体可用.
研究的目的:
- 开发和描述针对ZIKV EDIII的新型纳米体.
- 评估这些纳米体对ZIKV的中和能力.
- 根据ZIKV表观样本识别潜在的疫苗候选人.
主要方法:
- 一个天真的菌体库被用来衍生15个纳米体对抗ZIKV EDIII.
- 在体外选择中确定了高亲和度纳米体 (Nb-C9,Nb-D8,Nb-E3,Nb-E5) 与纳米分子范围内的解离常数.
- 进行了斑块减少中和试验 (PRNT50),以评估中和功效.
主要成果:
- 四个特定的纳米体 (Nb-C9,Nb-D8,Nb-E3,Nb-E5) 被确定具有高亲和力.
- Nb-E3和Nb-E5显示出强大的中和ZIKV菌株GD01,具有较低的PRNT50值.
- 一个由Nb-E5识别的线性表位 (Z17-VGEKKITHHWHRS) 被确定为潜在的疫苗候选者.
结论:
- 成功开发了针对ZIKV EDIII的新型纳米体.
- Nb-E3和Nb-E5具有显著的ZIKV中和能力.
- 鉴定的表位对开发基于表位的ZIKV疫苗充满希望,为新的治疗策略做出贡献.
相关概念视频
Cell-mediated Immune Responses
Overview
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Defense Against Bacterial Pathogens
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Immune Response Against Viral Pathogens
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
The Antiviral System of Bacteria and Archaea: CRISPR
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.

