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阳性电荷对脂质体稳定性,吸收效率和对细胞的影响

Feng Zhao¹, Diksha Nayyar¹, Young Soo Hwang¹

¹Department of Nanomedicine & Drug Targeting, Groningen Research Institute of Pharmacy (GRIP), University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.

International journal of pharmaceutics

|June 22, 2025

概括

带正电荷的脂质体显示细胞吸收增加但稳定性降低. 优化充电密度平衡了吸收效率和安全性,揭示了蛋白质甘氨酸相互作用介导了两种充电类型的输送.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
细胞生物学细胞生物学

背景情况:

与中性或负电荷对应物相比,正电荷的纳米载体往往表现出增强的细胞吸收.
然而,正电荷的增加会导致稳定性低下和细胞毒性.

研究的目的:

研究不同正电荷密度对脂质体稳定性,毒性和细胞吸收效率的影响.
为了比较优化正电荷脂质体与负电荷脂质体的吸收机制和效率.

主要方法:

制备脂质体,使正电荷脂质的度逐渐增加.
评估血清中的脂质体稳定性,细胞毒性和吸收效率.
对负荷相反的脂质体的吸收机制进行比较分析,包括蛋白质甘氨酸相互作用和冠状蛋白吸附.

主要成果:

增加正电荷增强了脂质体的吸收,但导致血清凝聚和细胞毒性增加.
一种具有较低正电荷密度的配方表明稳定性和低毒性.
带正电荷的脂质体显示出比负电荷的脂质体更高的吸收效率,由蛋白质甘油相互作用和差异性血清蛋白吸附介导.

结论:

脂质体电荷密度极大地影响稳定性,毒性和吸收.
优化的正电荷脂质体提供了可接受的安全配置文件的改善细胞传递.
蛋白质甘氨酸相互作用是脂质体吸收的关键,无论电荷如何,但表面特性调节效率.

关键词:

脂质体稳定性脂质体稳定性有正电荷的脂质体具有正电荷.蛋白质冠状病毒的冠状病毒吸收效率的使用效率.吸收机制吸收机制

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