来自Lactiplantibacillus plantarum的工程乳酸脱酶,用于利用人工辅助因子与光催化再生
Feifan Luo1, Xiangyuan Gu1, Binhao Wang1
1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
International journal of biological macromolecules
|June 22, 2025
概括
合成尼古丁胺胺辅因子仿生学 (NCBs) 被改造为来自Lactiplantibacillus plantarum的乳酸脱酶 (LDH). 这些经过修改的酶使用NCB有效地产生d-乳酸,显示出对工业应用的希望.
科学领域:
- 生物催化剂是一种生物催化剂.
- 酵素工程是什么? 酶工程是什么
- 合成生物学 合成生物学
背景情况:
- 乳酸脱酶 (LDH) 对于微生物的d-乳酸生产至关重要.
- 自然的尼古丁胺合因子 (NAD+) 在体外酶体过程中面临局限性.
- 合成尼古丁胺胺辅因子仿生学 (NCBs) 提供了潜在的替代品.
研究的目的:
- 为利用合成 NCB 设计 LpLDH.
- 调查NCB认可的结构性基础.
- 为了证明工程LpLDH在合光酶d-乳酸合成系统中的应用.
主要方法:
- 蛋白质工程和LpLDH的定向进化.
- 对于使用NCB变体的高通量选.
- 结构分析 (分子对接,分子动力学).
- 酶分析和光催化辅助因子再生.
主要成果:
- 已经确定了8种能够利用NCB的LpLDH变体.
- 变异P85I显示了与P2NAH和BNAH的增强活性,归因于额外的基-π相互作用.
- 结合的光酶合成实现了50%以上的转换,使用了NCB.
- 国家央行在高温下表现出比NAD+更高的稳定性和性能.
结论:
- 工程LpLDH有效地利用合成NCB进行d-乳酸生产.
- 合成NCB为生物催化剂中的天然辅助因子提供了强大而稳定的替代品.
- 这种方法对可持续的有机酸合成具有重大潜力.
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