来自Tridacna crocea的一种含有C1q域的新型蛋白质在共生体识别和免疫防御方面表现出双重功能
Wenjie Yi1, Yanna Tang1, Md Abu Kawsar2
1State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China; Sanya National Marine Ecosystem Research Station, Tropical Marine Biological Research Station in Hainan, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Chinese Academy of Sciences, Sanya, 572000, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
研究人员在巨中发现了一种新的C1q域含有 (C1qDC) 蛋白,TCC1qDC. 这种蛋白质识别了共生藻类和病原体,突出了其在共生和免疫防御中的双重作用.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 含C1q域 (C1qDC) 蛋白质作为模式识别受体,调解宿主-微生物相互作用.
- 这些受体利用它们的C端C1q域进行识别.
研究的目的:
- 为了表征一种来自Tridacna crocea的新型C1qDC蛋白,命名为TcC1qDC.
- 研究TcC1qDC在宿主微生物相互作用中的结合能力和功能作用.
主要方法:
- 来自Tridacna crocea的TcC1qDC的基因测序和蛋白质表征.
- 在不同组织中分析TcC1qDC表达模式.
- 在体外结合测定与共生性恐龙和微生物碳水化合物.
- 分子对接以预测碳水化合物识别.
- 测试与病原体相关的分子模式和微生物的结合活性.
- 评估微生物凝结能力.
主要成果:
- TcC1qDC是一种新型C1qDC蛋白,在Tridacna crocea中被发现.
- TcC1qDC是构成性表达的,在外围地幔和肝中表达的最高水平.
- 这种蛋白质与共生性恐龙鞭状体结合,并识别各种微生物碳水化合物.
- TcC1qDC表现出对PAMP和微生物的宽谱结合,包括格拉姆阴性细菌,格拉姆阳性细菌和真菌.
- 观察到显著的微生物聚合活性.
结论:
- 在巨型鱼中,TCC1qDC充当双受体.
- 它在弥合共生体的识别和免疫防御机制的激活方面发挥着至关重要的作用.
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