Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same author

Protocol for Nanodisc single-molecule pull-down assay to detect protein-lipid interactions.

STAR protocols·2026
Same author

Threonyl-tRNA synthetase activates STAT3 by a nontranslational mechanism.

The Journal of biological chemistry·2025
Same author

Regulation of Rho guanine nucleotide exchange factor 3 by phosphorylation in the PH domain.

iScience·2025
Same author

Leucyl-tRNA Synthetase Contributes to Muscle Weakness through Mammalian Target of Rapamycin Complex 1 Activation and Autophagy Suppression in a Mouse Model of Duchenne Muscular Dystrophy.

The American journal of pathology·2024
Same author

A non-translational role of threonyl-tRNA synthetase in regulating JNK signaling during myogenic differentiation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2021
Same journal

Erratum to 'Stearoyl-CoA desaturase-1 is vital for milk lipid synthesis: Deletion impairs mammary gland and neonatal development' [Journal of Lipid Research 66/12 (2025) 100941].

Journal of lipid research·2026
Same journal

The Metabolic-Immune Axis in MASH: Is the Macrophage Signature the Key to Liver Disease Staging?

Journal of lipid research·2026
Same journal

BK channel deficiency promotes lipolysis via an AKT-independent activation of the cAMP/PKA/HSL pathway.

Journal of lipid research·2026
Same journal

Interleukin-6 stimulates platelet 12-lipoxygenase to drive coagulation in inflammatory arthritis.

Journal of lipid research·2026
Same journal

LIPA regulates myeloid differentiation and is essential for intra-plaque macrophage accumulation during atherogenesis.

Journal of lipid research·2026
Same journal

Hydrolysis and depletion of phosphatidylglycerol at peak murine acute lung injury.

Journal of lipid research·2026
查看所有相关文章

相关实验视频

Updated: Sep 18, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.4K

纳米光盘单分子拉下来研究脂质-蛋白质相互作用.

Adriana Reyes-Ordoñez1, Shweta Shree2, Nilmani Singh3

  • 1Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Journal of lipid research
|June 22, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种基于纳米磁盘的单分子拉向 (SiMPull) 试验,以研究蛋白质-脂质相互作用. 这种方法使用纳米光盘来实现真正的单分子分辨率,揭示了AKT信号传输的关键相互作用.

关键词:
在 AKT AKT 里面.纳米光盘是一个纳米盘.SiMPull可以拉动.脂质脂质是什么意思 脂质脂质是什么意思酸是酸盐中的一种.一个单分子分子.

更多相关视频

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Published on: November 30, 2022

3.1K
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

25.8K

相关实验视频

Last Updated: Sep 18, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.4K
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Published on: November 30, 2022

3.1K
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Published on: August 31, 2012

25.8K

科学领域:

  • 生物化学 生物化学
  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 脂物,包括酸丁酸 (PIPs),是调节细胞过程的关键信号分子.
  • 研究特定的PIP蛋白相互作用对于理解细胞信号来说至关重要.
  • 使用小单状囊泡的现有方法在膜建模方面存在局限性.

研究的目的:

  • 开发一种强大且方便的方法,以单分子分辨率研究蛋白质脂质相互作用.
  • 通过使用纳米光盘作为膜模型来改善以前的脂质-SiMPull测定的局限性.
  • 研究AKT信号通路相互作用的结构基础.

主要方法:

  • 基于纳米盘的脂质单分子拉下测试 (纳米盘SiMPull) 的开发.
  • 使用含有各种PIP的纳米光盘捕获光标记蛋白质.
  • 分析蛋白质结合动力学,包括解离速率 (koff),使用停留时间分析.

主要成果:

  • 纳米磁盘SiMPull测定专门拉下PIP结合蛋白,检测值为10-20μM.
  • 试验实现了真正的单分子分辨率,每个纳米盘中只有一种蛋白质结合.
  • 确定了一种关键的分子内相互作用来稳定AKT与PI的结合{3,4,5) P3.3.

结论:

  • 纳米光盘SiMPull测定是一种强大的工具,用于研究单个分子水平的蛋白质-脂质相互作用.
  • 这种方法克服了以前基于囊泡的测试的局限性,提供了更准确的膜模型.
  • 提供了对管理AKT信号通路调节的结构机制的新见解.